School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing, 211800, China.
School of Pharmaceutical Sciences, Xiamen University, Xiamen, 361005, China.
Sci Rep. 2022 Oct 6;12(1):16689. doi: 10.1038/s41598-022-20648-z.
Alloferon-1 have been proposed as an effective peptide to enhance antitumoral immunity, antiviral defense and anti-inflammatory activity. This work aimed to assess anti-inflammatory effects of alloferon-1 against acute inflammation and histopathological deformations in λ-carrageenan-induced paw edema in mice. Systemic pretreatment with alloferon-1 (22.0 mg/kg) intraperitoneally injected mice showed a significant reduction in paw thickness and vascular permeability. Alloferon-1 prevented λ-carrageenan-evoked exudation and the neutrophil influx to the mouse pleura and the neutrophil migration into carrageenan-stimulated mouse air pouches based on the histopathological changes in the paw tissues. Administration of alloferon-1 also suppressed the expression of the inflammatory cytokines in the inflamed paw tissues such as tumor necrosis factor-α (TNF-α), monocyte chemoattractant protein 1 (MCP1), interleukin-5 (IL-5), etc. detected by Luminex liquid chip. Collectively, the present study provides evidences for the marked anti-inflammatory effects of alloferon-1 which might represent new therapeutic options for the treatment of acute inflammatory diseases.
阿洛福韦-1 被提议作为一种有效的肽，以增强抗肿瘤免疫、抗病毒防御和抗炎活性。本研究旨在评估 alloferon-1 对急性炎症和 λ-卡拉胶诱导的小鼠足肿胀的组织病理学改变的抗炎作用。全身预先给予 alloferon-1（22.0mg/kg）腹腔注射可显著减少爪厚度和血管通透性。alloferon-1 可预防 λ-卡拉胶诱导的渗出物和中性粒细胞向小鼠胸膜的浸润以及中性粒细胞向卡拉胶刺激的小鼠气囊的迁移，基于爪组织的组织病理学变化。alloferon-1 的给药还抑制了炎症性细胞因子在炎性爪组织中的表达，如肿瘤坏死因子-α（TNF-α）、单核细胞趋化蛋白 1（MCP1）、白细胞介素-5（IL-5）等，通过 Luminex 液体芯片检测。总之，本研究为 alloferon-1 的显著抗炎作用提供了证据，这可能为治疗急性炎症性疾病提供新的治疗选择。
