遗传性视网膜母细胞瘤患者及其家族成纤维细胞的X射线敏感性
X-ray sensitivity of fibroblasts from patients with hereditary retinoblastoma and their families.
作者信息
Pledger J V, Craft A W, Bartlett K, Long D R
出版信息
Br J Cancer. 1987 Jun;55(6):617-21. doi: 10.1038/bjc.1987.125.
The in vitro response to X-irradiation of cultured human fibroblasts was studied using a colony forming assay. A comprehensive reference range was established, giving a median D0 value of 98.5 cGy with an interquartile range of 86.5-110.5 cGy. Cells from 3 retinoblastoma family pedigrees were studied and the cell survival after exposure to X-rays was compared between affected (11 samples) and unaffected (26 samples) family members. No significant differences in response to ionising radiation were found between the controls, the affected and the unaffected members of the 3 families. The affected members had a median D0 of 97.5 cGy (interquartile range 87.5-107.5 cGy) and the unaffected members had a median D0 of 102 cGy (interquartile range 93-111 cGy). Thus radiosensitivity is not a useful marker for the detection of the retinoblastoma gene.
采用集落形成试验研究了培养的人成纤维细胞对X射线的体外反应。建立了一个全面的参考范围，D0值中位数为98.5 cGy，四分位间距为86.5 - 110.5 cGy。研究了来自3个视网膜母细胞瘤家族谱系的细胞，并比较了受影响（11个样本）和未受影响（26个样本）家族成员在X射线照射后的细胞存活率。在3个家族的对照组、受影响成员和未受影响成员之间，未发现对电离辐射的反应有显著差异。受影响成员的D0中位数为97.5 cGy（四分位间距87.5 - 107.5 cGy），未受影响成员的D0中位数为102 cGy（四分位间距93 - 111 cGy）。因此，放射敏感性不是检测视网膜母细胞瘤基因的有用标志物。
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