Biobehavioral Branch, Intramural Research Program, NINR, NIH, DHHS, Bethesda, MD 20892, USA.
Clin Chim Acta. 2013 Mar 15;418:97-101. doi: 10.1016/j.cca.2012.12.032. Epub 2013 Jan 14.
Abnormal gastrointestinal permeability has been linked to irritable bowel syndrome (IBS). The lactulose-to-mannitol ratio is traditionally used to assess small intestine permeability while sucralose and sucrose are used to assess colonic and gastric permeability respectively. We used a single 4-probe test solution to assess permeability throughout the gastrointestinal tract in IBS patients and healthy controls by measuring the recovery of the probes in urine after ingestion using a modified liquid chromatography mass spectrometry protocol.
Fasting participants (N=59) drank a permeability test solution (100ml: sucralose, sucrose, mannitol, and lactulose). Urine was collected over a 5-h period and kept frozen until analysis. Urinary sugar concentrations were measured using a liquid chromatography/triple quadruple mass spectrometer.
Colonic permeability was significantly lower in IBS patients when compared to healthy controls (p=0.011). Gastric and small intestinal permeability did not significantly differ between the groups.
The study demonstrates the clinical potential of this non-invasive method for assessing alterations in gastrointestinal permeability in patients with IBS.
胃肠道通透性异常与肠易激综合征（IBS）有关。传统上，乳果糖-甘露醇比值用于评估小肠通透性，而使用三氯蔗糖和蔗糖分别评估结肠和胃的通透性。我们使用单一的 4 探头测试溶液，通过使用改良的液相色谱-质谱联用协议测量摄入后尿液中探针的恢复情况，在 IBS 患者和健康对照组中评估整个胃肠道的通透性。
禁食参与者（N=59）饮用通透性测试溶液（100ml：三氯蔗糖、蔗糖、甘露醇和乳果糖）。在 5 小时内收集尿液，并在分析前冷冻保存。使用液相色谱/三重四极杆质谱仪测量尿糖浓度。
与健康对照组相比，IBS 患者的结肠通透性显著降低（p=0.011）。两组间胃和小肠通透性无显著差异。
该研究表明，这种非侵入性方法在评估 IBS 患者胃肠道通透性改变方面具有临床潜力。
