Department of Restorative Dentistry- Dental Materials Area, Piracicaba Dental School. State University of Campinas - UNICAMP, S.P., Brazil.
Department of Mechanical and Materials Engineering, Military Institute of Engineering- IME, Rio de Janeiro, R.J., Brazil; Department of Bioengineering, R-Crio Criogenia S.A., Campinas, S.P., Brazil.
J Mech Behav Biomed Mater. 2022 Dec;136:105464. doi: 10.1016/j.jmbbm.2022.105464. Epub 2022 Sep 24.
To develop a chitosan-xanthan (CX) membrane associated with Hydroxyapatite (HA) and different concentrations of graphene oxide (GO).
The CX complex was associated with the hydroxyapatite-graphene oxide (HAGO) nanocomposite in different concentrations. The experimental groups were:1) CX; 2) Chitosan-Xanthan/Hydroxyapatite (CXHA); 3) Chitosan-Xanthan/Hydroxyapatite-Graphene Oxide 0.5% (CXHAGO 0.5%); 4) CXHAGO 1.0%; 5) CXHAGO 1.5%. The membranes characterizations were performed by X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Raman spectroscopy, Scanning Electron Microscopy (SEM), Contact angle, Tensile Strength, in vitro Bioactivity and the in vitro Cell viability (MTT test). The data was submitted to the Normality and Homogeneity tests. In vitro Indirect Cytotoxicity assay data was statistically analyzed by two-way ANOVA and Tukey's test (α = 0.05). Tensile Strength and Contact Angle data were statistically analyzed by one-way ANOVA followed by Tukey's test (α = 0.05).
XRD, FTIR and Raman spectroscopy confirmed the characteristic bands of the CX polymeric complex, the phosphate bands related to HA, and the presence of GO. SEM images demonstrated the non-porous and homogeneous surface of membranes. The contact angle test showed the hydrophilic characteristic of all membranes (p > 0.05). CX showed tensile strength significantly higher than other membranes. The apatite deposition was observed in all membranes after performing the bioactivity test. The cell viability of CXHAGO 1.0% and CXHAGO 1.5% was significantly higher than CX.
The addition of HAGO reduced the mechanical strength of membranes, but improved its cell viability. It demonstrated the potential of CXHAGO membranes to be used in guided bone regeneration therapies.
开发一种与羟磷灰石（HA）和不同浓度氧化石墨烯（GO）相关的壳聚糖-黄原胶（CX）膜。
将 CX 复合物与羟磷灰石-氧化石墨烯（HAGO）纳米复合材料以不同浓度结合。实验组为：1）CX；2）壳聚糖-黄原胶/羟磷灰石（CXHA）；3）壳聚糖-黄原胶/羟磷灰石-氧化石墨烯 0.5%（CXHAGO 0.5%）；4）CXHAGO 1.0%；5）CXHAGO 1.5%。通过 X 射线衍射（XRD）、傅里叶变换红外光谱（FTIR）、拉曼光谱、扫描电子显微镜（SEM）、接触角、拉伸强度、体外生物活性和体外细胞活力（MTT 试验）对膜进行表征。数据接受正态性和同质性检验。通过双因素方差分析和 Tukey 检验（α=0.05）对体外间接细胞毒性试验数据进行统计学分析。拉伸强度和接触角数据通过单因素方差分析和 Tukey 检验（α=0.05）进行统计学分析。
XRD、FTIR 和拉曼光谱证实了 CX 聚合物复合物的特征带、与 HA 相关的磷酸盐带和 GO 的存在。SEM 图像显示膜的非多孔和均匀表面。接触角试验表明所有膜均具有亲水性特征（p>0.05）。CX 的拉伸强度明显高于其他膜。在进行生物活性试验后，所有膜均观察到有磷灰石沉积。CXHAGO 1.0%和 CXHAGO 1.5%的细胞活力明显高于 CX。
HAGO 的添加降低了膜的机械强度，但提高了其细胞活力。这证明了 CXHAGO 膜在引导骨再生治疗中的应用潜力。
