Liu Shun, Storti Mattia, Finazzi Giovanni, Bowler Chris, Dorrell Richard G
Institut de Biologie de l'Ecole Normale Supérieure (IBENS), Ecole Normale Supérieure, Centre National De La Recherche Scientifique (CNRS), Institut National De La Santé Et De La Recherche Médicale (INSERM), Université Paris Sciences et Lettres (PSL), Paris, France.
CNRS Research Federation for the study of Global Ocean Systems Ecology and Evolution, FR2022/Tara Oceans GOSEE, 3 rue Michel-Ange, Paris, France.
Front Plant Sci. 2022 Sep 22;13:950467. doi: 10.3389/fpls.2022.950467. eCollection 2022.
Diatoms are an important group of algae, contributing nearly 40% of total marine photosynthetic activity. However, the specific molecular agents and transporters underpinning the metabolic efficiency of the diatom plastid remain to be revealed. We performed analyses of 70 predicted plastid transporters identified by genome-wide searches of . We considered similarity with plastid transporters, transcriptional co-regulation with genes encoding core plastid metabolic pathways and with genes encoded in the mitochondrial genomes, inferred evolutionary histories using single-gene phylogeny, and environmental expression trends using Oceans meta-transcriptomics and meta-genomes data. Our data reveal diatoms conserve some of the ion, nucleotide and sugar plastid transporters associated with plants, such as non-specific triose phosphate transporters implicated in the transport of phosphorylated sugars, NTP/NDP and cation exchange transporters. However, our data also highlight the presence of diatom-specific transporter functions, such as carbon and amino acid transporters implicated in intricate plastid-mitochondria crosstalk events. These confirm previous observations that substrate non-specific triose phosphate transporters (TPT) may exist as principal transporters of phosphorylated sugars into and out of the diatom plastid, alongside suggesting probable agents of NTP exchange. Carbon and amino acid transport may be related to intricate metabolic plastid-mitochondria crosstalk. We additionally provide evidence from environmental meta-transcriptomic/meta- genomic data that plastid transporters may underpin diatom sensitivity to ocean warming, and identify a diatom plastid transporter (J43171) whose expression may be positively correlated with temperature.
硅藻是一类重要的藻类，贡献了近40%的海洋总光合活性。然而，支撑硅藻质体代谢效率的具体分子因子和转运蛋白仍有待揭示。我们对通过全基因组搜索鉴定出的70种预测质体转运蛋白进行了分析。我们考虑了与其他质体转运蛋白的相似性、与编码核心质体代谢途径的基因以及线粒体基因组中编码的基因的转录共调控，利用单基因系统发育推断进化历史，并利用海洋元转录组学和元基因组数据研究环境表达趋势。我们的数据显示，硅藻保留了一些与植物相关的离子、核苷酸和糖质体转运蛋白，例如参与磷酸化糖转运的非特异性磷酸丙糖转运蛋白、NTP/NDP和阳离子交换转运蛋白。然而，我们的数据也突出了硅藻特异性转运蛋白功能的存在，例如参与复杂的质体 - 线粒体相互作用事件的碳和氨基酸转运蛋白。这些证实了先前的观察结果，即底物非特异性磷酸丙糖转运蛋白（TPT）可能作为磷酸化糖进出硅藻质体的主要转运蛋白存在，同时暗示了NTP交换的可能因子。碳和氨基酸转运可能与复杂的质体 - 线粒体代谢相互作用有关。我们还从环境元转录组学/元基因组数据中提供证据，表明质体转运蛋白可能是硅藻对海洋变暖敏感性的基础，并鉴定出一种硅藻质体转运蛋白（J43171），其表达可能与温度呈正相关。
