• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

温氏解毒方通过重塑肠道微生物群和肿瘤微环境来改善结直肠癌。

Wenzi Jiedu Recipe ameliorates colorectal cancer by remodeling the gut microbiota and tumor microenvironment.

作者信息

Qiu Wenli, Sang Tianqing, Chen Haibin, Zhou Hongli, Wang Zhongqiu, Zhou Hongguang

机构信息

Department of Radiology, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, China.

The First Clinical Medical College, Henan University of Chinese Medicine, Zhengzhou, China.

出版信息

Front Oncol. 2022 Sep 23;12:915498. doi: 10.3389/fonc.2022.915498. eCollection 2022.

DOI:10.3389/fonc.2022.915498
PMID:36212428
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9541612/
Abstract

INTRODUCTION

Wenzi Jiedu Recipe (WJR), traditional Chinese medicine (TCM) formula, has been proven to be clinically useful in the treatment of colorectal cancer (CRC). However, its underlying mechanisms are still elusive, which limits its wider application. Thus, we aimed to evaluate the effect of WJR on CRC and elucidate mechanisms underlying its action.

METHODS

Network pharmacology was employed to clarify the "herb-active ingredient-target" network of WJR. The 16S rDNA sequencing method was used to analyze the changes of gut microbes mediated by WJR in tumor-bearing mice with CRC. The proportions of CD4+ T cell and CD8+ T cell were measured by flow cytometry. Levels of the cytokines interleukin (IL)-10, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were assessed by immunohistochemistry and enzyme-linked immunosorbent assay (ELISA).

RESULTS

WJR showed significant anti-CRC effects both and . Network pharmacology revealed that WJR exerts anti-CRC therapeutic effect on multiple targets and signaling pathways. Gut microbiota analysis revealed that WJR therapy significantly enriched for and . In particular, we found that WJR significantly increased the proportion of CD8+ T cells and the expression of immune-associated cytokines IL-10, IFN-γ, and TNF-α.

CONCLUSION

The regulation of gut microbiota by WJR may be the breakthrough point to clarify its mechanism of action in the treatment of CRC, and it has a good prospect of clinical application.

摘要

引言

中药方剂文子解毒方（WJR）已被证明在治疗结直肠癌（CRC）方面具有临床疗效。然而，其潜在机制仍不清楚，这限制了其更广泛的应用。因此，我们旨在评估WJR对CRC的作用，并阐明其作用的潜在机制。

方法

采用网络药理学方法阐明WJR的“草药-活性成分-靶点”网络。利用16S rDNA测序方法分析WJR对荷瘤CRC小鼠肠道微生物的影响。通过流式细胞术检测CD4+ T细胞和CD8+ T细胞的比例。采用免疫组织化学和酶联免疫吸附测定（ELISA）法评估细胞因子白细胞介素（IL）-10、干扰素（IFN）-γ和肿瘤坏死因子（TNF）-α的水平。

结果

WJR在[具体方面1]和[具体方面2]均显示出显著的抗CRC作用。网络药理学研究表明，WJR对多个靶点和信号通路发挥抗CRC治疗作用。肠道微生物群分析显示，WJR治疗显著富集了[具体微生物群1]和[具体微生物群2]。特别是，我们发现WJR显著增加了CD8+ T细胞的比例以及免疫相关细胞因子IL-10、IFN-γ和TNF-α的表达。

结论

WJR对肠道微生物群的调节可能是阐明其在CRC治疗中作用机制的突破点，具有良好的临床应用前景。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f8/9541612/30c59ca4f173/fonc-12-915498-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f8/9541612/e784cdaf9fd2/fonc-12-915498-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f8/9541612/8d379979ccac/fonc-12-915498-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f8/9541612/3fcdc2490665/fonc-12-915498-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f8/9541612/58ca743befe9/fonc-12-915498-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f8/9541612/30c59ca4f173/fonc-12-915498-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f8/9541612/e784cdaf9fd2/fonc-12-915498-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f8/9541612/8d379979ccac/fonc-12-915498-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f8/9541612/3fcdc2490665/fonc-12-915498-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f8/9541612/58ca743befe9/fonc-12-915498-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f8/9541612/30c59ca4f173/fonc-12-915498-g005.jpg

相似文献

1
Wenzi Jiedu Recipe ameliorates colorectal cancer by remodeling the gut microbiota and tumor microenvironment.温氏解毒方通过重塑肠道微生物群和肿瘤微环境来改善结直肠癌。
Front Oncol. 2022 Sep 23;12:915498. doi: 10.3389/fonc.2022.915498. eCollection 2022.
2
Integrated gut microbiota and metabolome analysis reveals the mechanism of Xiaoai Jiedu recipe in ameliorating colorectal cancer.整合肠道微生物群和代谢组分析揭示消癌解毒方改善结直肠癌的机制。
Front Oncol. 2023 Jun 22;13:1184786. doi: 10.3389/fonc.2023.1184786. eCollection 2023.
3
Gegen Qinlian decoction enhances immunity and protects intestinal barrier function in colorectal cancer patients gut microbiota.葛根芩连汤增强结直肠癌患者肠道微生物群的免疫力并保护肠道屏障功能。
World J Gastroenterol. 2020 Dec 28;26(48):7633-7651. doi: 10.3748/wjg.v26.i48.7633.
4
YYFZBJS ameliorates colorectal cancer progression in Apc mice by remodeling gut microbiota and inhibiting regulatory T-cell generation.YYFZBJS 通过重塑肠道微生物群和抑制调节性 T 细胞生成来改善 Apc 小鼠的结直肠癌进展。
Cell Commun Signal. 2020 Jul 16;18(1):113. doi: 10.1186/s12964-020-00596-9.
5
Pectin supplement significantly enhanced the anti-PD-1 efficacy in tumor-bearing mice humanized with gut microbiota from patients with colorectal cancer.果胶补充剂显著增强了用来自结直肠癌患者的肠道微生物群人源化的荷瘤小鼠的抗PD-1疗效。
Theranostics. 2021 Feb 19;11(9):4155-4170. doi: 10.7150/thno.54476. eCollection 2021.
6
Jianpi Jiedu Recipe inhibits colorectal cancer liver metastasis via regulating ITGBL1-rich extracellular vesicles mediated activation of cancer-associated fibroblasts.健脾解毒方通过调节富含整合素β样蛋白1的细胞外囊泡介导的癌症相关成纤维细胞激活来抑制结直肠癌肝转移。
Phytomedicine. 2022 Jun;100:154082. doi: 10.1016/j.phymed.2022.154082. Epub 2022 Mar 28.
7
JianPiTongLuo (JPTL) Recipe regulates anti-apoptosis and cell proliferation in colorectal cancer through the PI3K/AKT signaling pathway.健脾通络（JPTL）方通过PI3K/AKT信号通路调节结直肠癌中的抗凋亡和细胞增殖。
Heliyon. 2024 Jul 31;10(15):e35490. doi: 10.1016/j.heliyon.2024.e35490. eCollection 2024 Aug 15.
8
Pharmacological Mechanism of Pingxiao Formula against Colorectal Cancer.平消方抗结直肠癌的药理机制
Evid Based Complement Alternat Med. 2022 Dec 20;2022:7884740. doi: 10.1155/2022/7884740. eCollection 2022.
9
Sini Decoction Inhibits Tumor Progression and Enhances the Anti-Tumor Immune Response in a Murine Model of Colon Cancer.四逆汤抑制结肠癌荷瘤小鼠肿瘤进展并增强抗肿瘤免疫应答。
Comb Chem High Throughput Screen. 2023;26(14):2517-2526. doi: 10.2174/1386207326666230320103437.
10
Probiotic powder ameliorates colorectal cancer by regulating Bifidobacterium animalis, Clostridium cocleatum, and immune cell composition.益生菌粉通过调节动物双歧杆菌、柯林斯氏菌和免疫细胞组成来改善结直肠癌。
PLoS One. 2023 Mar 13;18(3):e0277155. doi: 10.1371/journal.pone.0277155. eCollection 2023.

引用本文的文献

1
Role of gut microbiome in suppression of cancers.肠道微生物群在癌症抑制中的作用。
Gut Microbes. 2025 Dec;17(1):2495183. doi: 10.1080/19490976.2025.2495183. Epub 2025 Apr 20.
2
The colorectal cancer microenvironment: Preclinical progress in identifying targets for cancer therapy.结直肠癌微环境：癌症治疗靶点识别的临床前进展
Transl Oncol. 2025 Mar;53:102307. doi: 10.1016/j.tranon.2025.102307. Epub 2025 Feb 3.
3
Potential of natural products and gut microbiome in tumor immunotherapy.天然产物与肠道微生物群在肿瘤免疫治疗中的潜力。

本文引用的文献

1
Role of the gut microbiota in airway immunity and host defense against respiratory infections.肠道微生物群在气道免疫和宿主防御呼吸道感染中的作用。
Biol Chem. 2021 Oct 4;402(12):1481-1491. doi: 10.1515/hsz-2021-0281. Print 2021 Nov 25.
2
Fecal Bacteria as Non-Invasive Biomarkers for Colorectal Adenocarcinoma.粪便细菌作为结直肠癌的非侵入性生物标志物
Front Oncol. 2021 Aug 10;11:664321. doi: 10.3389/fonc.2021.664321. eCollection 2021.
3
Efficacy of transcatheter arterial chemoembolization combined with capecitabine and cetuximab in the treatment of colorectal cancer with liver metastasis.
Chin Med. 2024 Nov 20;19(1):161. doi: 10.1186/s13020-024-01032-7.
4
Application of omics technologies in studies on antitumor effects of Traditional Chinese Medicine.组学技术在中医药抗肿瘤作用研究中的应用
Chin Med. 2024 Sep 9;19(1):123. doi: 10.1186/s13020-024-00995-x.
5
Natural Shikonin Potentially Alters Intestinal Flora to Alleviate Acute Inflammation.天然紫草素可能通过改变肠道菌群来减轻急性炎症。
Microorganisms. 2023 Aug 23;11(9):2139. doi: 10.3390/microorganisms11092139.
6
Integrated gut microbiota and metabolome analysis reveals the mechanism of Xiaoai Jiedu recipe in ameliorating colorectal cancer.整合肠道微生物群和代谢组分析揭示消癌解毒方改善结直肠癌的机制。
Front Oncol. 2023 Jun 22;13:1184786. doi: 10.3389/fonc.2023.1184786. eCollection 2023.
经导管动脉化疗栓塞联合卡培他滨和西妥昔单抗治疗结直肠癌肝转移的疗效。
J BUON. 2021 May-Jun;26(3):1002-1008.
4
Sini Decoction Ameliorates Colorectal Cancer and Modulates the Composition of Gut Microbiota in Mice.四逆汤改善小鼠结直肠癌并调节肠道微生物群组成。
Front Pharmacol. 2021 Mar 11;12:609992. doi: 10.3389/fphar.2021.609992. eCollection 2021.
5
Herbal medicine WangShiBaoChiWan improves gastrointestinal health in mice via modulation of intestinal tight junctions and gut microbiota and inhibition of inflammation.中药王氏保赤丸通过调节肠道紧密连接和肠道微生物群及抑制炎症来改善小鼠的胃肠道健康。
Biomed Pharmacother. 2021 Jun;138:111426. doi: 10.1016/j.biopha.2021.111426. Epub 2021 Mar 21.
6
The colorectal cancer-associated faecal microbiome of developing countries resembles that of developed countries.发展中国家结直肠癌相关粪便微生物组与发达国家相似。
Genome Med. 2021 Feb 16;13(1):27. doi: 10.1186/s13073-021-00844-8.
7
Cancer Statistics, 2021.癌症统计数据，2021.
CA Cancer J Clin. 2021 Jan;71(1):7-33. doi: 10.3322/caac.21654. Epub 2021 Jan 12.
8
Xiaoai Jiedu Recipe suppresses hepatocellular carcinogenesis through the miR-200b-3p /Notch1 axis.消癌解毒方通过miR-200b-3p/Notch1轴抑制肝细胞癌发生。
Cancer Manag Res. 2020 Nov 2;12:11121-11131. doi: 10.2147/CMAR.S269991. eCollection 2020.
9
β-Sitosterol Reverses Multidrug Resistance via BCRP Suppression by Inhibiting the p53-MDM2 Interaction in Colorectal Cancer.β-谷甾醇通过抑制结直肠癌中 p53-MDM2 相互作用来逆转多药耐药，BCRP。
J Agric Food Chem. 2020 Mar 25;68(12):3850-3858. doi: 10.1021/acs.jafc.0c00107. Epub 2020 Mar 13.
10
New Insights for Cellular and Molecular Mechanisms of Aging and Aging-Related Diseases: Herbal Medicine as Potential Therapeutic Approach.衰老及衰老相关疾病的细胞和分子机制的新见解：草药作为潜在的治疗方法。
Oxid Med Cell Longev. 2019 Dec 12;2019:4598167. doi: 10.1155/2019/4598167. eCollection 2019.