Wang Zhangjun, Li Qingfeng, Liu Caixia, Liu Fenglou, Xu Nali, Yao Mingming, Yu Huixia, Wang Yanqing, Chen Jiajing, Bai Shuangyu, Yang Jingxin, Sun Gang, Long Jiaohui, Fan Yalei, Kang Ling, Li Hongxia, Zhang Xiaogang, Liu Shengxiang
Nanjing Agricultural University, Cytogenetics Institute, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing, Jiangsu, China.
Ningxia University, School of Agriculture, Yinchuan, Ningxia, China.
Genet Mol Biol. 2022 Oct 7;45(3):e20220117. doi: 10.1590/1678-4685-GMB-2022-0117. eCollection 2022.
Hordeum californicum (H. californicum, 2n=2X=14, HcHc), one of the wild relatives of wheat (Triticum aestivum L.), harbors many desirable genes and is a potential genetic resource for wheat improvement. In this study, an elite line ND646 was selected from a BC4F5 population, which was developed using 60Co-γ irradiated wheat-H. californicum disomic addition line WJ28-1 (DA6Hc) as the donor parent and Ningchun 4 as the recurrent parent. ND646 was identified as a novel wheat-H. californicum 6HcS/6BL translocation line using genomic in situ hybridization (GISH), fluorescence in situ hybridization (FISH), and H. californicum-specific expressed sequence tag (EST) markers. Further evaluation revealed that ND646 had excellent performance in several traits, such as a higher sedimentation value (SV), higher water absorption rate (WAR), and higher hardness index (HI). More importantly, it had more kernels per spike (KPS), a higher grain yields (GY), and good resistance to powdery mildew, leaf rust, and 2,4-D butylate (2,4-D). Its excellent phenotypic performance laid the foundation for further investigation of its genetic architecture and makes ND646 a useful germplasm resource for wheat breeding.
加州大麦（Hordeum californicum，2n = 2X = 14，HcHc）是小麦（Triticum aestivum L.）的野生近缘种之一，含有许多优良基因，是小麦改良的潜在遗传资源。在本研究中，从一个BC4F5群体中选育出一个优良品系ND646，该群体是以60Co-γ辐照的小麦-加州大麦二体附加系WJ28-1（DA6Hc）为供体亲本、宁春4号为轮回亲本培育而成。利用基因组原位杂交（GISH）、荧光原位杂交（FISH）和加州大麦特异性表达序列标签（EST）标记，将ND646鉴定为一个新型的小麦-加州大麦6HcS/6BL易位系。进一步评价发现，ND646在多个性状上表现优异，如沉降值（SV）较高、吸水率（WAR）较高和硬度指数（HI）较高。更重要的是，它每穗粒数（KPS）更多、籽粒产量（GY）较高，对白粉病、叶锈病和2,4-滴丁酯（2,4-D）具有良好抗性。其优异的表型表现为进一步研究其遗传结构奠定了基础，并使ND646成为小麦育种的有用种质资源。
