Rashed Saba Sadeghi, Ghaffari Mehran, Moghadam Nahid Beladi, Ebrahimi Maryam Tajabadi, Keshtmand Zahra
Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
Department of Neurology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Metab Brain Dis. 2022 Dec;37(8):2777-2782. doi: 10.1007/s11011-022-01092-4. Epub 2022 Oct 10.
Cadmium (Cd) is a toxicant metal that risks human and animal health. Nowadays, the vital role of Aquaporin-4 (AQP-4) in brain and gut cell permeability has gathered too much attention to protecting against heavy metals. Studies have shown that heavy metals can harm the body due to oxidative stress. Probiotics are known for their health-beneficial effects and establish as dietary adjuncts mainly for their antioxidant properties. This study investigated the impact of a novel probiotic combination including Lactobacillus casei IBRC-M10783, Lactobacillus rhamnosus IBRC-M10782, and Lactobacillus helveticus TG-34 on the AQP-4 gene expression in CdCl2-induced Wistar rats. Rats were divided into three groups and received a specific dose of CdCl2 or probiotics. The AQP-4 expression level had estimated by Real-Time PCR in both the intestine and brain. These results showed a significant reduction in AQP-4 gene expression in the probiotic treatment group compared to the CdCl2 control group in the intestine and brain for the first time. Our research showed that consuming a probiotic mixture of L. casei, L. rhamnosus, and L. helveticus can reduce the expression of the aquaporin-4 gene in the brain and intestine of rats exposed to Cadmium, which can be promising in the field of aquaporin-4 regulation.
镉（Cd）是一种有毒金属，会危害人类和动物健康。如今，水通道蛋白4（AQP - 4）在脑和肠道细胞通透性方面的重要作用已备受关注，这对于抵御重金属至关重要。研究表明，重金属可因氧化应激而损害身体。益生菌因其有益健康的作用而闻名，主要因其抗氧化特性而被确立为膳食辅助剂。本研究调查了一种新型益生菌组合，包括干酪乳杆菌IBRC - M10783、鼠李糖乳杆菌IBRC - M10782和瑞士乳杆菌TG - 34对氯化镉诱导的Wistar大鼠中AQP - 4基因表达的影响。将大鼠分为三组，并给予特定剂量的氯化镉或益生菌。通过实时定量聚合酶链反应（Real - Time PCR）对肠道和脑中的AQP - 4表达水平进行了评估。这些结果首次表明，与氯化镉对照组相比，益生菌治疗组在肠道和脑中的AQP - 4基因表达显著降低。我们的研究表明，食用干酪乳杆菌、鼠李糖乳杆菌和瑞士乳杆菌的益生菌混合物可降低镉暴露大鼠脑和肠道中水通道蛋白4基因的表达，这在水通道蛋白4调节领域可能具有前景。
