Zhai Qixiao, Tian Fengwei, Zhao Jianxin, Zhang Hao, Narbad Arjan, Chen Wei
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China UK-China Joint Centre on Probiotic Bacteria, Norwich, United Kingdom.
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.
Appl Environ Microbiol. 2016 Jun 30;82(14):4429-40. doi: 10.1128/AEM.00695-16. Print 2016 Jul 15.
The heavy metal cadmium (Cd) is an environmental pollutant that causes adverse health effects in humans and animals. Our previous work demonstrated that oral administration of probiotics can significantly inhibit Cd absorption in the intestines of mice, but further evidence is needed to gain insights into the related protection mode. The goal of this study was to evaluate whether probiotics can inhibit Cd absorption through routes other than the Cd binding, with a focus on gut barrier protection. In the in vitro assay, both the intervention and therapy treatments of Lactobacillus plantarum CCFM8610 alleviated Cd-induced cytotoxicity in the human intestinal cell line HT-29 and protected the disruption of tight junctions in the cell monolayers. In a mouse model, probiotics with either good Cd-binding or antioxidative ability increased fecal Cd levels and decreased Cd accumulation in the tissue of Cd-exposed mice. Compared with the Cd-only group, cotreatment with probiotics also reversed the disruption of tight junctions, alleviated inflammation, and decreased the intestinal permeability of mice. L. plantarum CCFM8610, a strain with both good Cd binding and antioxidative abilities, exhibited significantly better protection than the other two strains. These results suggest that along with initial intestinal Cd sequestration, probiotics can inhibit Cd absorption by protecting the intestinal barrier, and the protection is related to the alleviation of Cd-induced oxidative stress. A probiotic with both good Cd-binding and antioxidative capacities can be used as a daily supplement for the prevention of oral Cd exposure.
The heavy metal cadmium (Cd) is an environmental pollutant that causes adverse health effects in humans and animals. For the general population, food and drinking water are the main sources of Cd exposure due to the biomagnification of Cd within the food chain; therefore, the intestinal tract is the first organ that is susceptible to Cd contamination. Moreover, Cd exposure causes the disruption of the intestinal barrier and further induces the amplification of Cd absorption. The present study confirms that, along with initial intestinal Cd sequestration, oral administration of probiotics can inhibit Cd absorption by protecting the intestinal barrier. A probiotic with both good Cd-binding and antioxidative capacities can be used as a daily supplement for the prevention of oral Cd exposure.
重金属镉（Cd）是一种环境污染物，会对人类和动物的健康产生不利影响。我们之前的研究表明，口服益生菌可显著抑制小鼠肠道对镉的吸收，但需要更多证据来深入了解相关的保护模式。本研究的目的是评估益生菌是否能通过除镉结合之外的途径抑制镉吸收，重点是肠道屏障保护。在体外试验中，植物乳杆菌CCFM8610的干预和治疗处理均减轻了镉诱导的人肠道细胞系HT - 29的细胞毒性，并保护了细胞单层紧密连接的破坏。在小鼠模型中，具有良好镉结合或抗氧化能力的益生菌增加了粪便中镉的水平，并降低了镉暴露小鼠组织中的镉积累。与仅镉处理组相比，益生菌联合处理还逆转了紧密连接的破坏，减轻了炎症，并降低了小鼠的肠道通透性。植物乳杆菌CCFM8610，一种具有良好镉结合和抗氧化能力的菌株，表现出比其他两种菌株更好的保护作用。这些结果表明，除了最初在肠道中螯合镉外，益生菌还可通过保护肠道屏障来抑制镉吸收，且这种保护作用与减轻镉诱导的氧化应激有关。一种具有良好镉结合和抗氧化能力的益生菌可作为日常补充剂用于预防口服镉暴露。
重金属镉（Cd）是一种环境污染物，会对人类和动物的健康产生不利影响。对于普通人群而言，由于镉在食物链中的生物放大作用导致食物和饮用水是镉暴露的主要来源；因此，肠道是最易受镉污染的首个器官。此外，镉暴露会导致肠道屏障破坏，并进一步诱导镉吸收的增加。本研究证实，除了最初在肠道中螯合镉外，口服益生菌可通过保护肠道屏障来抑制镉吸收。一种具有良好镉结合和抗氧化能力的益生菌可作为日常补充剂用于预防口服镉暴露。
