埃塞俄比亚患有一系列肝脏疾病的慢性乙肝携带者中丁型肝炎病毒的流行程度和基因型

Magnitude and genotype of hepatitis delta virus among chronic hepatitis B carriers with a spectrum of liver diseases in Ethiopia.

作者信息

Tassachew Yayehyirad, Belyhun Yeshambel, Abebe Tamrat, Mihret Adane, Teffera Tezazu, Ababi Girma, Shewaye Abate, Desalegn Hailemichael, Aseffa Abraham, Mulu Andargachew, Howe Rawleigh, Liebert Uwe G, Maier Melanie

机构信息

Department of Microbiology, Immunology, and Parasitology, School of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia; Institute of Virology, University of Leipzig, 04103 Leipzig, Germany; Armauer Hansen Research Institute (AHRI), Addis Ababa, Ethiopia; School of Medicine, College of Medicine and Health Sciences, Hawassa University, Hawassa, Ethiopia.

Institute of Virology, University of Leipzig, 04103 Leipzig, Germany; School of Biomedical and Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia.

出版信息

Ann Hepatol. 2023 Jan-Feb;28(1):100770. doi: 10.1016/j.aohep.2022.100770. Epub 2022 Oct 8.

DOI:10.1016/j.aohep.2022.100770

PMID:36220615

Abstract

INTRODUCTION AND OBJECTIVES

Chronic hepatitis D infection contributes substantially to the progression of chronic liver disease, especially in most low and middle-income countries, where hepatitis B virus-related chronic liver disease is endemic. Therefore, this study aimed to determine the magnitude and genotype of hepatitis delta virus (HDV) among patients with chronic hepatitis B (CHB)-related liver diseases in Ethiopia.

PATIENTS AND METHODS

In this cross-sectional study, 323 known HBsAg positive individuals comprising 220 patients with CHB-related liver diseases [121 advanced liver diseases (hepatocellular carcinoma /HCC/ and non-HCC) and 99 chronic hepatitis (CH)], and 103 symptomless blood donors (BD) were enrolled. An ELISA kit was employed to determine HDV infection, and quantitative real-time PCR was used to detect HDV RNA. In addition, a non-coding genomic RNA region was sequenced for genotyping and phylogenetic analysis.

RESULTS

Irrespective of the stage of liver disease, the overall magnitude of HDV was 7.7% (25/323). The frequency of anti-HDV increases with the severity of liver disease, 1.9%, 4%, 10%, and 21.3% among BD, CH, non-HCC, and HCC patients, respectively. HDV RNA has been detected in 1.54 %(5/323) cases with a mean viral load of 4,010,360 IU/ml. All isolates were found to be HDV genotype 1.

CONCLUSIONS

The magnitude of HDV infection increased with the severity of liver disease, indicating HDV infection is more common among patients with CHB-related liver diseases in Ethiopia.

摘要

引言与目的

慢性丁型肝炎感染在慢性肝病进展中起重要作用，尤其在大多数乙肝病毒相关慢性肝病流行的低收入和中等收入国家。因此，本研究旨在确定埃塞俄比亚慢性乙型肝炎（CHB）相关肝病患者中丁型肝炎病毒（HDV）的感染率及基因型。

患者与方法

在这项横断面研究中，纳入了323名已知HBsAg阳性个体，其中包括220例CHB相关肝病患者[121例晚期肝病（肝细胞癌/HCC/和非HCC）和99例慢性肝炎（CH）]，以及103名无症状献血者（BD）。采用ELISA试剂盒检测HDV感染情况，并用定量实时PCR检测HDV RNA。此外，对一个非编码基因组RNA区域进行测序以进行基因分型和系统发育分析。

结果

无论肝病处于何种阶段，HDV的总体感染率为7.7%（25/323）。抗HDV的频率随肝病严重程度增加，在BD、CH、非HCC和HCC患者中的比例分别为1.9%、4%、10%和21.3%。在1.54%（5/323）的病例中检测到HDV RNA，平均病毒载量为4,010,360 IU/ml。所有分离株均为HDV基因型1。