State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China.
Environ Sci Technol. 2022 Nov 15;56(22):15869-15881. doi: 10.1021/acs.est.2c03675. Epub 2022 Oct 13.
As an emerging two-dimensional nanomaterial with promising prospects, mono- or few-layer black phosphorus (BP) is potentially toxic to humans. We investigated the effects of two types of BPs on adult male mice through intratracheal instillation. Using the flow cytometry method, the generation, migration, and recruitment of immune cells in different organs have been characterized on days 1, 7, 14, and 21 post-exposure. Compared with small BP (S-BP, lateral size at ∼188 nm), large BP (L-BP, lateral size at ∼326 nm) induced a stronger stress lymphopoiesis and B cell infiltration into the alveolar sac. More importantly, L-BP dramatically increased peripheral neutrophil (NE) counts up to 1.9-fold on day 21 post-exposure. Decreased expression of the CXCR4 on NEs, an important regulator of NE retention in the bone marrow, explained the increased NE release into the circulation induced by L-BP. Therefore, BP triggers systemic inflammation via the disruption of both the generation and migration of inflammatory immune cells.
作为一种具有广阔前景的新兴二维纳米材料，单层或少层黑磷（BP）对人类具有潜在毒性。我们通过气管内滴注研究了两种 BP 对成年雄性小鼠的影响。使用流式细胞术方法，在暴露后第 1、7、14 和 21 天，对不同器官中免疫细胞的生成、迁移和募集进行了表征。与小 BP（S-BP，横向尺寸约为 188nm）相比，大 BP（L-BP，横向尺寸约为 326nm）诱导更强的应激淋巴生成和 B 细胞浸润到肺泡囊中。更重要的是，L-BP 在暴露后第 21 天显著增加外周血中性粒细胞（NE）计数高达 1.9 倍。NE 上 CXCR4 的表达降低，这是 NE 保留在骨髓中的重要调节因子，解释了 L-BP 诱导的 NE 向循环系统的释放增加。因此，BP 通过破坏炎症免疫细胞的生成和迁移引发全身炎症。
