Ji Yang, Hou Mengdie, Zhang Jin, Jin Meiqi, Wang Tianlin, Yang Huazhe, Zhang Xiaodong
Department of Stomatology, General Hospital of Northern Theater Command, Shenyang 110016, China.
School of Intelligent Medicine, China Medical University, Shenyang 110122, China.
Polymers (Basel). 2022 Oct 9;14(19):4233. doi: 10.3390/polym14194233.
In the field of bone repair, the inorganic-organic composite scaffold is a promising strategy for mimicking the compositions of the natural bone. In addition, as implants for repairing load-bearing sites, an inert permanent bone substitute composites with bioactive degradable ingredients may make full use of the composite scaffold. Herein, the porous zirconia (ZrO) matrix was prepared via the template replication method, and the partial degradable ZrO-chitosan particles-GelMA composite scaffolds with different chitosan/GelMA volume ratios were prepared through the vacuum infiltration method. Dynamic light scattering (DLS) and the scanning electron microscope (SEM) were adopted to observe the size of the chitosan particles and the morphologies of the composites scaffold. The mechanical properties, swelling properties, and degradation properties of the composite scaffolds were also characterized by the mechanical properties testing machine and immersion tests. The CCK-8 assay was adopted to test the biocompatibility of the composite scaffold preliminarily. The results show that chitosan particles as small as 60 nm were obtained. In addition, the ratio of chitosan/GelMA can influence the mechanical properties and the swelling and degradation behaviors of the composites scaffold. Furthermore, improved cell proliferation performance was obtained for the composite scaffolds.
在骨修复领域，无机-有机复合支架是一种很有前景的模拟天然骨成分的策略。此外，作为修复承重部位的植入物，一种含有生物活性可降解成分的惰性永久性骨替代复合材料可能会充分利用复合支架。在此，通过模板复制法制备了多孔氧化锆（ZrO）基体，并通过真空浸渍法制备了具有不同壳聚糖/甲基丙烯酸缩水甘油酯（GelMA）体积比的部分可降解ZrO-壳聚糖颗粒-GelMA复合支架。采用动态光散射（DLS）和扫描电子显微镜（SEM）观察壳聚糖颗粒的尺寸和复合支架的形貌。还通过力学性能测试机和浸泡试验对复合支架的力学性能、溶胀性能和降解性能进行了表征。采用CCK-8法初步测试了复合支架的生物相容性。结果表明，获得了尺寸小至60 nm的壳聚糖颗粒。此外，壳聚糖/GelMA的比例会影响复合支架的力学性能以及溶胀和降解行为。此外，复合支架的细胞增殖性能得到了改善。
