Biological Mimetics, Inc., Frederick, MD, United States.
Wound Infections Department, Bacterial Diseases Branch, Center for Infectious Disease Research, Walter Reed Army Institute of Research, Silver Spring, MD, United States.
Front Immunol. 2022 Sep 27;13:941010. doi: 10.3389/fimmu.2022.941010. eCollection 2022.
causes multi-system diseases in both nosocomial settings and a pre-disposed general population. The bacterium is not only desiccation-resistant but also notoriously resistant to multiple antibiotics and drugs of last resort including carbapenem, colistin, and sulbactam. The World Health Organization has categorized carbapenem-resistant at the top of its critical pathogen list in a bid to direct urgent countermeasure development. Several early-stage vaccines have shown a range of efficacies in healthy mice, but no vaccine candidates have advanced into clinical trials. Herein, we report our findings that both an ionizing γ-radiation-inactivated and a non-ionizing ultraviolet C-inactivated whole-cell vaccine candidate protects neutropenic mice from pulmonary challenge with virulent AB5075, a particularly pathogenic isolate. In addition, we demonstrate that a humoral response is sufficient for this protection the passive immunization of neutropenic mice.
导致医院环境和易感染人群发生多系统疾病。该细菌不仅具有抗干燥能力，而且对多种抗生素和最后手段的药物（包括碳青霉烯类、黏菌素和舒巴坦）具有明显的耐药性。世界卫生组织已将耐碳青霉烯类肠杆菌科细菌列为其关键病原体清单的首位，旨在指导紧急对策的制定。几种早期疫苗在健康小鼠中显示出了不同的疗效，但没有疫苗候选者进入临床试验。在此，我们报告发现，经电离γ 射线灭活和非电离紫外线 C 灭活的全细胞候选疫苗均能保护中性粒细胞减少症小鼠免受强毒 AB5075 的肺部攻击，AB5075 是一种特别具有致病性的分离株。此外，我们还证明了体液反应足以提供这种保护——中性粒细胞减少症小鼠的被动免疫。
