Mukai Kanae, Komatsu Emi, Yamanishi Misa, Hutakuchi Misako, Kanzaka Kayo, Uno Yuka, Yamazaki Shizuka, Kato Shizuka, Yamamoto Tomomi, Hattori Mayumi, Nakajima Yukari, Urai Tamae, Asano Kimi, Murakado Naoko, Okuwa Mayumi, Nakatani Toshio
Wounds. 2017 Jan;29(1):1-9. Epub 2016 Oct 24.
The aim of this study was to investigate the effects of changing the application of Japanese honey to a hydrocolloid dressing (HCD) in between the inflammatory and proliferative phases on cutaneous wound healing in 8-week-old, BALB/cCrSlc male mice.
Mice were divided into 4 groups: acacia honey followed by a HCD, buckwheat flour honey followed by a HCD, Chinese milk vetch honey followed by a HCD, and a HCD alone (control group). All mice received 2 full-thickness wounds on both sides of the dorsum using a Disposable Biopsy Punch. The wounds of the control group were covered with a HCD, whereas wounds in the other groups were treated with 0.1 mL of the relevant type of honey until day 3 post-wound and then were covered with a HCD from days 4 to 14.
In the experimental groups, the wound area ratio was significantly smaller in the inflammatory phase but significantly larger in the proliferative phase. Reepithelialization, collagen deposition, and wound contraction were significantly delayed compared with those in the control group.
The re-expansion of the wounds in the proliferative phase could not be prevented, and reepithelialization, collagen deposition, and wound contraction were delayed compared with those upon the use of a HCD.
The study's authors concluded that these methods do not promote cutaneous wound healing better than the use of a HCD alone.
本研究的目的是调查在8周龄的BALB/cCrSlc雄性小鼠的皮肤伤口愈合过程中，在炎症期和增殖期之间将日本蜂蜜应用于水胶体敷料（HCD）的效果。
将小鼠分为4组：刺槐蜂蜜后接HCD组、荞麦蜂蜜后接HCD组、紫云英蜂蜜后接HCD组和仅使用HCD组（对照组）。所有小鼠均使用一次性活检打孔器在背部两侧造成2个全层伤口。对照组的伤口用HCD覆盖，而其他组的伤口在伤口后第3天用0.1 mL相应类型的蜂蜜处理，然后在第4天至第14天用HCD覆盖。
在实验组中，伤口面积比在炎症期显著较小，但在增殖期显著较大。与对照组相比，再上皮化、胶原蛋白沉积和伤口收缩明显延迟。
增殖期伤口的再次扩张无法预防，与使用HCD相比，再上皮化、胶原蛋白沉积和伤口收缩延迟。
该研究的作者得出结论，这些方法并不比单独使用HCD更能促进皮肤伤口愈合。
