Luxembourg Institute of Science and Technology (LIST), Environmental Research & Innovation Department, 41 rue du Brill, L-4422 Belvaux, Luxembourg.
Luxembourg Institute of Science and Technology (LIST), Environmental Research & Innovation Department, 41 rue du Brill, L-4422 Belvaux, Luxembourg.
Sci Total Environ. 2023 Jan 20;857(Pt 2):159401. doi: 10.1016/j.scitotenv.2022.159401. Epub 2022 Oct 12.
Understanding the persistence of SARS-CoV-2 biomarkers in wastewater should guide wastewater-based epidemiology users in selecting best RNA biomarkers for reliable detection of the virus during current and future waves of the pandemic. In the present study, the persistence of endogenous SARS-CoV-2 were assessed during one month for six different RNA biomarkers and for the pepper mild mottle virus (PMMoV) at three different temperatures (4, 12 and 20 °C) in one wastewater sample. All SARS-CoV-2 RNA biomarkers were consistently detected during 6 days at 4° and differences in signal persistence among RNA biomarkers were mostly observed at 20 °C with N biomarkers being globally more persistent than RdRP, E and ORF1ab ones. SARS-CoV-2 signal persistence further decreased in a temperature dependent manner. At 12 and 20 °C, RNA biomarker losses of 1-log occurred on average after 6 and 4 days, and led to a complete signal loss after 13 and 6 days, respectively. Besides the effect of temperature, SARS-CoV-2 RNA signals were more persistent in the particulate phase compared to the aqueous one. Finally, PMMoV RNA signal was highly persistent in both phases and significantly differed from that of SARS-CoV-2 biomarkers. We further provide a detailed overview of the latest literature on SARS-CoV-2 and PMMoV decay rates in sewage matrices.
了解 SARS-CoV-2 生物标志物在废水中的持久性，应指导基于废水的流行病学用户选择最佳 RNA 生物标志物，以便在当前和未来的大流行浪潮中可靠检测病毒。在本研究中，在一个废水样本中，在 4、12 和 20°C 三个不同温度下，评估了六种不同的 RNA 生物标志物和辣椒轻斑驳病毒 (PMMoV) 中内源性 SARS-CoV-2 的持久性。在 4°C 下，所有 SARS-CoV-2 RNA 生物标志物在 6 天内均被持续检测到，而在 20°C 下，RNA 生物标志物之间的信号持久性差异最大，N 生物标志物的整体持久性强于 RdRP、E 和 ORF1ab 生物标志物。SARS-CoV-2 信号的持久性进一步随温度的降低而降低。在 12 和 20°C 时，RNA 生物标志物的损失平均在 6 和 4 天后发生 1 个对数，分别导致 13 和 6 天后完全信号丢失。除了温度的影响外，SARS-CoV-2 RNA 信号在颗粒相中的持久性比水相中的持久性更强。最后，PMMoV RNA 信号在两相中都具有很高的持久性，并且与 SARS-CoV-2 生物标志物的信号有明显差异。我们进一步提供了关于 SARS-CoV-2 和 PMMoV 在污水基质中衰减率的最新文献的详细概述。
