Tortolini Cristina, Angeloni Antonio, Antiochia Riccarda
Department of Experimental Medicine University of Rome "La Sapienza" Viale Regina Elena 324 00166 Rome Italy.
Department of Chemistry and Drug Technologies University of Rome "La Sapienza" P.le Aldo Moro 5 00185 Rome Italy.
Electroanalysis. 2022 Sep 29. doi: 10.1002/elan.202200349.
Here, a novel biosensing platform for the detection of SARS-CoV-2 usable both at voltammetric and impedimetric mode is reported. The platform was constructed on a multi-walled carbon nanotubes (MWCNTs) screen-printed electrode (SPE) functionalized by methylene blue (MB), antibodies against SARS-CoV-2 spike protein (SP), a bioactive layer of chitosan (CS) and protein A (PrA). The voltammetric sensor showed superior performances both in phosphate buffer solution (PBS) and spiked-saliva samples, with LOD values of 5.0±0.1 and 30±2.1 ng/mL, compared to 20±1.8 and 50±2.5 ng/mL for the impedimetric sensor. Moreover, the voltammetric immunosensor was tested in real saliva, showing promising results.
本文报道了一种新型生物传感平台，可用于伏安法和阻抗法检测严重急性呼吸综合征冠状病毒2（SARS-CoV-2）。该平台构建在多壁碳纳米管（MWCNTs）丝网印刷电极（SPE）上，该电极由亚甲蓝（MB）、抗SARS-CoV-2刺突蛋白（SP）抗体、壳聚糖（CS）生物活性层和蛋白A（PrA）功能化。伏安传感器在磷酸盐缓冲溶液（PBS）和加标唾液样品中均表现出优异性能，检测限（LOD）值分别为5.0±0.1和30±2.1 ng/mL，而阻抗传感器的LOD值分别为20±1.8和50±2.5 ng/mL。此外，伏安免疫传感器在实际唾液中进行了测试，结果令人满意。
