Department of Chemistry, Faculty of Sciences, Ilam University, P.O. Box 69315-516, Ilam, Iran.
Mikrochim Acta. 2022 Jul 19;189(8):287. doi: 10.1007/s00604-022-05357-8.
A dual recognition biosensor was developed via introducing aptamer strings and molecular imprinting polymer (MIP) for the selective detection of intact SARS-CoV-2 virus based on screen printed carbon electrode (SPCE) modified with nickel-benzene tricarboxylic acid-metal-organic framework (Ni(BTC) MOF) synthesized by in situ growth method, SARS-CoV-2 S protein-specific amino-aptamer and electropolymerization of dopamine (ePDA). The proposed biosensor showed an excellent linear relationship between charge transfer resistance (R) and increase in virus concentration in the range 10 to 10 plaque-forming units/mL (PFU/mL) with a low detection limit of 3.3 ± 0.04 PFU/mL and response time of 20 min. Compared with single-element sensors (aptamer or MIP), it showed higher selectivity for the SARS-CoV-2 virus and facilitated detection in real samples.
通过引入适体链和分子印迹聚合物（MIP），开发了一种双重识别生物传感器，该传感器基于通过原位生长法合成的镍-苯三甲酸-金属有机骨架（Ni（BTC）MOF）修饰的丝网印刷碳电极（SPCE），用于选择性检测完整的 SARS-CoV-2 病毒，SARS-CoV-2 S 蛋白特异性氨基适体和多巴胺的电聚合（ePDA）。所提出的生物传感器在电荷转移电阻（R）和病毒浓度增加之间显示出出色的线性关系，其检测范围为 10 至 10 噬菌斑形成单位/mL（PFU/mL），检测限低至 3.3±0.04 PFU/mL，响应时间为 20 分钟。与单元素传感器（适体或 MIP）相比，它对 SARS-CoV-2 病毒表现出更高的选择性，并有利于实际样品的检测。
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