Zhang Xiaorui, Lv Hongyang, Tian Maoying, Dong Zhaowei, Fu Qinwen, Sun Jilin, Huang Qinwan, Wang Jin
State Key Laboratory of Characteristic Chinese Medicine Resources in Southwest China, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Front Plant Sci. 2022 Sep 28;13:984483. doi: 10.3389/fpls.2022.984483. eCollection 2022.
, is a plant of the Persicaria genus, which is commonly used to treat various diseases, including gastrointestinal disorders, neurological disorders, inflammation, and diarrhea. However, because of different local standards of , people often confuse it with L. and other closely related plants. This poses a serious threat to the safety and efficacy of the clinical use of . This study aims to determine the six active ingredients of and . Then the endophytic fungi and rhizosphere soil of the two species were sequenced by Illumina Miseq PE300. The results show significant differences between the community composition of the leaves, stems, and roots of the and the in the same soil environment. Of the six secondary metabolites detected, five had significant differences between and . Then, we evaluated the composition of the significantly different communities between and . In the , the relative abundance of differential communities in the leaves was highest, of which dominated the differential communities in the leaves and stem; in the , the relative abundance of differential community in the stem was highest, and dominated the differential communities in the three compartments. By constructing the interaction network of and and analyzing the network nodes, we found that the core community in accounted for 87.59% of the total community, dominated by ; the core community of accounted for 19.81% of the total community, dominated by . Of these core communities, 23 were significantly associated with active ingredient content. Therefore, we believe that the community from significantly interferes with recruiting fungal communities in and affects the accumulation of secondary metabolites in the host plant. These results provide an essential foundation for the large-scale production of . They indicate that by colonizing specific fungal communities, secondary metabolic characteristics of host plants can be helped to be shaped, which is an essential means for developing new medicinal plants.
[植物名称]是蓼属植物，常用于治疗各种疾病，包括胃肠道疾病、神经系统疾病、炎症和腹泻。然而，由于[植物名称]的地方标准不同，人们常常将它与[另一种植物名称]L.及其他近缘植物混淆。这对[植物名称]临床应用的安全性和有效性构成了严重威胁。本研究旨在确定[植物名称]和[另一种植物名称]的六种活性成分。然后，通过Illumina Miseq PE300对这两个物种的内生真菌和根际土壤进行测序。结果表明，在相同土壤环境下，[植物名称]和[另一种植物名称]的叶、茎和根的群落组成存在显著差异。在检测到的六种次生代谢产物中，[植物名称]和[另一种植物名称]之间有五种存在显著差异。然后，我们评估了[植物名称]和[另一种植物名称]之间显著不同群落的组成。在[植物名称]中，叶中差异群落的相对丰度最高，其中[某种真菌名称]在叶和茎的差异群落中占主导地位；在[另一种植物名称]中，茎中差异群落的相对丰度最高，[另一种真菌名称]在三个部位的差异群落中占主导地位。通过构建[植物名称]和[另一种植物名称]的相互作用网络并分析网络节点，我们发现[植物名称]中的核心群落占总群落的87.59%，以[某种真菌名称]为主导；[另一种植物名称]的核心群落占总群落的19.81%，以[另一种真菌名称]为主导。在这些核心群落中，有23个与活性成分含量显著相关。因此，我们认为[植物名称]的群落显著干扰了[另一种植物名称]中真菌群落的招募，并影响了宿主植物中次生代谢产物的积累。这些结果为[植物名称]的大规模生产提供了重要基础。它们表明，通过定殖特定的真菌群落，可以帮助塑造宿主植物的次生代谢特征，这是开发新药用植物的重要手段。
