Colonization characteristics of fungi in L. and L. and its effect on the content of active ingredients.

, is a plant of the Persicaria genus, which is commonly used to treat various diseases, including gastrointestinal disorders, neurological disorders, inflammation, and diarrhea. However, because of different local standards of , people often confuse it with L. and other closely related plants. This poses a serious threat to the safety and efficacy of the clinical use of . This study aims to determine the six active ingredients of and . Then the endophytic fungi and rhizosphere soil of the two species were sequenced by Illumina Miseq PE300. The results show significant differences between the community composition of the leaves, stems, and roots of the and the in the same soil environment. Of the six secondary metabolites detected, five had significant differences between and . Then, we evaluated the composition of the significantly different communities between and . In the , the relative abundance of differential communities in the leaves was highest, of which dominated the differential communities in the leaves and stem; in the , the relative abundance of differential community in the stem was highest, and dominated the differential communities in the three compartments. By constructing the interaction network of and and analyzing the network nodes, we found that the core community in accounted for 87.59% of the total community, dominated by ; the core community of accounted for 19.81% of the total community, dominated by . Of these core communities, 23 were significantly associated with active ingredient content. Therefore, we believe that the community from significantly interferes with recruiting fungal communities in and affects the accumulation of secondary metabolites in the host plant. These results provide an essential foundation for the large-scale production of . They indicate that by colonizing specific fungal communities, secondary metabolic characteristics of host plants can be helped to be shaped, which is an essential means for developing new medicinal plants.