Khattab Marwa S, Aly Tahany A A, Mohamed Sara M, Naguib Abdel Moneim M, Al-Farga Ammar, Abdel-Rahim Emam A
Pathology Department, Faculty of Veterinary Medicine Cairo University Giza Egypt.
Regional Center for Food and Feed, Agriculture Research Center, Ministry of Agriculture Giza Egypt.
Food Sci Nutr. 2022 Jul 7;10(10):3355-3367. doi: 10.1002/fsn3.2936. eCollection 2022 Oct.
Diabetes mellitus type 2 (DM) is a common chronic disease worldwide, which may be due to increased environmental pollution. Aflatoxin B1 is a likely inevitable contaminant in food and dairy products. Both DM and aflatoxicosis exert a deleterious effect on reproduction urging the exploration of various functional food for protection. This study investigated the effect of barley microgreen (BM) on reproductive disorders caused by DM with or without aflatoxicosis in male rats. Rats were divided into eight groups; G1 control, G2 barley, G3 aflatoxin, G4 aflatoxin-barley, G5 streptozotocin (STZ), G6 STZ-barley, G7 STZ-aflatoxin, and G8 STZ-aflatoxin-barley. BM chemical composition revealed elevated calcium, iron, phosphorus, and vitamin A compared with barely seeds. Complete blood picture, lipid profile, serum oxidative stress parameters, relative testicular weight, sperm analysis, chromosomal aberration, and testis histopathology were performed. The lipid profile was altered significantly in G7. Oxidative stress was increased in G3, G5, and G7, whereas it was decreased in BM-treated groups. Sperm counts were reduced significantly in aflatoxin and/or STZ groups but increased significantly in BM-treated groups. Sperm morphological abnormalities and chromosomal aberrations were decreased significantly in BM-treated groups compared with untreated groups. Testicular histopathology revealed moderate diffuse degeneration of seminiferous tubules in aflatoxin and/or STZ groups, which were alleviated in BM-treated groups. In conclusion, aflatoxin and STZ together caused severe reproductive disorder and oxidative stress more than aflatoxin or STZ alone. BM diet reduced significantly oxidative stress and reproductive disorder associated with DM and aflatoxicosis in rats.
2型糖尿病（DM）是一种全球常见的慢性疾病，这可能归因于环境污染的加剧。黄曲霉毒素B1是食品和乳制品中可能不可避免的污染物。糖尿病和黄曲霉毒素中毒都会对生殖产生有害影响，促使人们探索各种功能性食品来进行保护。本研究调查了大麦嫩苗（BM）对雄性大鼠在患有或未患有黄曲霉毒素中毒的情况下由糖尿病引起的生殖障碍的影响。将大鼠分为八组：G1对照组、G2大麦组、G3黄曲霉毒素组、G4黄曲霉毒素-大麦组、G5链脲佐菌素（STZ）组、G6 STZ-大麦组、G7 STZ-黄曲霉毒素组和G8 STZ-黄曲霉毒素-大麦组。与大麦种子相比，BM的化学成分显示钙（Ca）、铁（Fe）、磷（P）和维生素A含量升高。进行了全血细胞计数、血脂谱、血清氧化应激参数、相对睾丸重量、精子分析、染色体畸变和睾丸组织病理学检查。G7组的血脂谱有显著改变。G3、G5和G7组的氧化应激增加，而在BM处理组中氧化应激降低。黄曲霉毒素和/或STZ组的精子计数显著减少，但BM处理组的精子计数显著增加。与未处理组相比，BM处理组的精子形态异常和染色体畸变显著减少。睾丸组织病理学显示，黄曲霉毒素和/或STZ组的生精小管有中度弥漫性变性，而在BM处理组中这种变性得到缓解。总之，黄曲霉毒素和STZ共同导致的严重生殖障碍和氧化应激比单独使用黄曲霉毒素或STZ时更严重。BM饮食显著降低了与大鼠糖尿病和黄曲霉毒素中毒相关的氧化应激和生殖障碍。
