Abdel-Latif Mohamed S, Elmeleigy Khaled M, Aly Tahany A A, Khattab Marwa S, Mohamed Sara M
Biochemistry Department, Faculty of Agriculture, Cairo University, Giza, Egypt.
Regional Center For Food and Feed, Agriculture Research Center, Ministry of Agriculture, Giza, Egypt.
Exp Toxicol Pathol. 2017 Jun 14;69(5):285-291. doi: 10.1016/j.etp.2017.01.014. Epub 2017 Feb 9.
Aflatoxin contamination of animal diet has adverse effects on animal health and productivity. This study was performed to investigate the effect of using coumarin and/or chlorophyllin in rat diet against aflatoxicosis. Fifty-four rats were assigned into 7 groups (6 rats each). G1 was a negative control. G2 received water with coumarin 0.5%. G3 received water with chlorophyllin 0.5%. G4 received water with coumarin 0.5% and chlorophyllin 0.5%. G5-8 fed aflatoxin B 1000ppb in diet. Group 6-8 were administered similar treatments as G2-4. The experiment ended after 8 weeks. Random glucose, total lipid, total cholesterol, total triglycerides, total protein, serum ALT, AST, creatinine, and urea were measured. Histopathology of liver, kidney and pancreas and immunohistochemical staining of placental glutathione-S-transferase (GST-P) in liver were performed. The glucose serum level, cholesterol, AST, and ALT were elevated in G5 compared to G6-8. The liver and kidney lesions in G5 included vacuolation and necrosis which subsided in G6-8. The necrosis and inflammatory cells infiltration in the pancreas of G5 were absent in G6-8. GST-P positive hepatocytes were abundant in G5, few in G6 and absent in G7 and G8. In conclusion, the chlorophyllin and coumarin possessed protective and anti-carcinogenic effect against aflatoxicosis in rats.
动物饲料中的黄曲霉毒素污染会对动物健康和生产力产生不利影响。本研究旨在调查在大鼠饲料中使用香豆素和/或叶绿酸对黄曲霉毒素中毒的影响。将54只大鼠分为7组（每组6只）。G1为阴性对照组。G2饮用含0.5%香豆素的水。G3饮用含0.5%叶绿酸的水。G4饮用含0.5%香豆素和0.5%叶绿酸的水。G5 - 8的饲料中含有1000 ppb的黄曲霉毒素B。第6 - 8组接受与G2 - 4相似的处理。8周后实验结束。测量随机血糖、总脂质、总胆固醇、总甘油三酯、总蛋白、血清谷丙转氨酶（ALT）、谷草转氨酶（AST）、肌酐和尿素。进行肝脏、肾脏和胰腺的组织病理学检查以及肝脏中胎盘谷胱甘肽 - S - 转移酶（GST - P）的免疫组化染色。与G6 - 8相比，G5组的血糖血清水平、胆固醇、AST和ALT升高。G5组的肝脏和肾脏病变包括空泡化和坏死，在G6 - 8组中减轻。G5组胰腺中的坏死和炎性细胞浸润在G6 - 8组中不存在。GST - P阳性肝细胞在G5组中大量存在,在G6组中较少，在G7和G8组中不存在。总之，叶绿酸和香豆素对大鼠黄曲霉毒素中毒具有保护和抗癌作用。
