Sitnikov Dmitry S, Revkova Veronika A, Ilina Inna V, Gurova Svetlana A, Komarov Pavel S, Struleva Evgenia V, Konoplyannikov Mikhail A, Kalsin Vladimir A, Baklaushev Vladimir P
Joint Institute for High Temperatures of the Russian Academy of Sciences, Moscow, Russia.
Federal Research and Clinical Center of Specialized Medical Care and Medical Technologies FMBA of Russia, Moscow, Russia.
J Biophotonics. 2023 Jan;16(1):e202200212. doi: 10.1002/jbio.202200212. Epub 2022 Oct 27.
The data is obtained on the effect of high-intensity pulses of terahertz (THz) radiation with a broad spectrum (0.2-3 THz) on cell cultures. We have evaluated the threshold exposure parameters of THz radiation causing genotoxic effects in fibroblasts. Phosphorylation of histone H2AX at Ser 139 (γH2AX) was chosen as a marker for genotoxicity and a quantitative estimation of γH2AX foci number in fibroblasts was performed after cell irradiation with THz pulses for 30 min. No genotoxic effects of THz radiation were observed in fibroblasts unless peak intensity and electric field strength exceeded 21 GW cm and 2.8 MV cm , respectively. In tumor cell lines (neuroblastoma (SK-N-BE (2)) and glioblastoma (U87)), exposure to THz pulses with peak intensity of 21 GW cm for 30 min caused no morphological changes as well as no statistically significant increase in histone phosphorylation foci number.
获取了关于具有宽频谱（0.2 - 3太赫兹）的太赫兹（THz）辐射高强度脉冲对细胞培养物影响的数据。我们评估了太赫兹辐射在成纤维细胞中引起遗传毒性效应的阈值暴露参数。选择组蛋白H2AX在丝氨酸139处的磷酸化（γH2AX）作为遗传毒性的标志物，并在用太赫兹脉冲照射细胞30分钟后，对成纤维细胞中γH2AX焦点数量进行了定量估计。除非峰值强度和电场强度分别超过21 GW/cm²和2.8 MV/cm，否则在成纤维细胞中未观察到太赫兹辐射的遗传毒性效应。在肿瘤细胞系（神经母细胞瘤（SK - N - BE(2)）和胶质母细胞瘤（U87））中，用峰值强度为21 GW/cm²的太赫兹脉冲照射30分钟，未引起形态学变化，组蛋白磷酸化焦点数量也未出现统计学上的显著增加。
