泰国口腔鳞状细胞癌患者的 Epstein-Barr 病毒检测和 LMP-1 表达。
Epstein-Barr Virus Detection and LMP-1 Expression in Thai Patients with Oral Squamous Cell Carcinoma.
机构信息
Department of Oral Medicine and Periodontology, Faculty of Dentistry, Mahidol University, Rajthewee, Bangkok, 10400, Thailand.
Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Mahidol University, Rajthewee, Bangkok, 10400, Thailand.
出版信息
Head Neck Pathol. 2023 Mar;17(1):210-217. doi: 10.1007/s12105-022-01501-1. Epub 2022 Oct 18.
BACKGROUND
To date, high-risk human papillomavirus (HPV) is primarily linked to oropharyngeal carcinoma, but only a small subset of oral squamous cell carcinoma (OSCC) is truly driven by high-risk HPV. In addition, Epstein-Barr virus (EBV) is another potential oncogenic virus for OSCC development. This study aims to investigate the role of EBV infection in Thai patients with OSCC.
METHODS
Forty-seven formalin-fixed paraffin-embedded specimens of OSCC were obtained. EBV DNA was detected by polymerase chain reaction analysis using primers for LMP-1 region of EBV. EBV-positive OSCC cases were subjected to LMP-1 immunohistochemical analysis and EBV-encoded small RNA (EBER) in situ hybridization to determine EBV cellular localization in OSCC. LMP-1 immunohistochemical analysis was also performed in all EBV-negative OSCC cases.
RESULTS
Of the 47 OSCC specimens, ten (21%) exhibited EBV DNA by PCR analysis. Seven of ten (70%) EBV-positive specimens showed high-grade LMP-1 expression by immunohistochemistry. However, no EBER expression was detected in all EBV-positive OSCC specimens. In EBV-negative specimens, LMP-1 was also negative except in 3 specimens which showed low grade expression of LMP-1.
CONCLUSION
The prevalence of EBV infection in OSCC in this group of Thai patients was 21%. Most of EBV-positive OSCC cases showed LMP-1 expression but a lack of EBER expression. From our findings, we presume that EBV may take some roles in OSCC development in this group of participants.
背景
迄今为止，高危型人乳头瘤病毒（HPV）主要与口咽癌相关，但仅有一小部分口腔鳞状细胞癌（OSCC）真正由高危 HPV 驱动。此外，EB 病毒（EBV）也是 OSCC 发展的另一种潜在致癌病毒。本研究旨在探讨 EBV 感染在泰国 OSCC 患者中的作用。
方法
获得 47 例福尔马林固定石蜡包埋的 OSCC 标本。采用 EBV LMP-1 区引物的聚合酶链反应分析检测 EBV DNA。对 EBV 阳性 OSCC 病例进行 LMP-1 免疫组织化学分析和 EBV 编码的小 RNA（EBER）原位杂交，以确定 EBV 在 OSCC 中的细胞定位。所有 EBV 阴性 OSCC 病例也进行了 LMP-1 免疫组织化学分析。
结果
在 47 例 OSCC 标本中，有 10 例（21%）通过 PCR 分析显示 EBV DNA。10 例 EBV 阳性标本中有 7 例（70%）通过免疫组化显示高水平 LMP-1 表达。然而，所有 EBV 阳性 OSCC 标本均未检测到 EBER 表达。在 EBV 阴性标本中，除 3 例 LMP-1 低水平表达外，LMP-1 也呈阴性。
结论
在这组泰国患者中，OSCC 中 EBV 感染的流行率为 21%。大多数 EBV 阳性 OSCC 病例显示 LMP-1 表达，但缺乏 EBER 表达。根据我们的发现，我们推测 EBV 可能在这组参与者的 OSCC 发展中起一定作用。
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