School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, People's Republic of China.
School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China.
Environ Monit Assess. 2022 Oct 18;194(12):921. doi: 10.1007/s10661-022-10606-7.
Pathogenic fungal infections in fruit cause economic losses and have deleterious effects on human health globally. Despite the low pH and high water contents of vegetables and fresh, ripened fruits, they are prone to fungal and bacterial diseases. The ever-increasing resistance of phytopathogens toward pesticides, fungicides and bactericides has resulted in substantial threats to plant growth and production in recent years. However, plant-mediated nanoparticles are useful tools for combating parasitic fungi and bacteria. Herein, we synthesized biogenic manganese oxide nanoparticles (MnONPs) from an extract of Punica granatum (P. granatum), and these nanoparticles showed significant antifungal and antibacterial activities. The production of MnONPs from plant extracts was confirmed by infrared spectroscopy (FTIR), X-ray diffraction (XRD) and UV visible spectroscopy (UV). The surface morphology and shape of the nanoparticles were characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Using a detached fruit method, the MnONPs were shown to exhibit significant antimicrobial activities against two bacterial strains, E. coli and S. aureus, and against the fungal species P. digitatum. The results revealed that the MnONPs had a minimum antimicrobial activity at 25 µg/mL and a maximum antimicrobial activity at 100 µg/mL against bacterial strains in lemon (citrus). Furthermore, the MnONPs exhibited significant ROS scavenging activity. Finally, inconclusive results from the green-synthesized MnONPs magnified their significant synergetic effects on the shelf life of tomatoes (Lycopercicum esculantum) and indicated that they could be used to counteract the phytopathological effects of postharvest fungal diseases in fruits and vegetables. Overall, this method of MnONPs synthesis is inexpensive, rapid and ecofriendly. MnONPs can be used as potential antimicrobial agents against different microbial species.
致病真菌感染水果会造成全球经济损失，并对人类健康产生有害影响。尽管蔬菜和新鲜成熟水果的 pH 值低、含水量高，但它们仍容易受到真菌和细菌疾病的影响。近年来，植物病原菌对杀虫剂、杀菌剂和杀细菌剂的抗性不断增加，对植物的生长和生产造成了重大威胁。然而，植物介导的纳米颗粒是对抗寄生真菌和细菌的有用工具。在此，我们从石榴（Punica granatum）的提取物中合成了生物源氧化锰纳米颗粒（MnONPs），这些纳米颗粒表现出显著的抗真菌和抗菌活性。通过红外光谱（FTIR）、X 射线衍射（XRD）和紫外可见光谱（UV）证实了植物提取物中 MnONPs 的产生。通过扫描电子显微镜（SEM）和透射电子显微镜（TEM）对纳米颗粒的表面形貌和形状进行了表征。使用离体果实法，MnONPs 对两种细菌菌株大肠杆菌和金黄色葡萄球菌以及真菌物种柑橘绿霉表现出显著的抗菌活性。结果表明，MnONPs 对柠檬（柑橘）中的细菌菌株在 25μg/mL 时具有最小的抗菌活性，在 100μg/mL 时具有最大的抗菌活性。此外，MnONPs 表现出显著的 ROS 清除活性。最后，由绿色合成的 MnONPs 产生的不确定结果放大了它们对番茄（Lycopercicum esculantum）货架期的显著协同作用，并表明它们可用于对抗水果和蔬菜收获后真菌病的植物病理效应。总的来说，这种 MnONPs 合成方法成本低廉、快速且环保。MnONPs 可作为针对不同微生物物种的潜在抗菌剂。
