Center for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Bellaterra, Barcelona, Spain.
Department of Biology, Healthcare and the Environment, Plant Physiology Section, Faculty of Pharmacy and Food Sciences, Universitat de Barcelona, Barcelona, Spain.
Int J Syst Evol Microbiol. 2022 Oct;72(10). doi: 10.1099/ijsem.0.005557.
In the framework of the research project called fitomatics, we have isolated and characterized a bacterial plant-endophyte from the rhizomes of , hereafter referred to as strain FIT81. The bacterium is Gram negative, rod-shaped with lophotrichous flagella, and catalase- and oxidase-positive. The optimal growth temperature of strain FIT81 is 28 °C, although it can grow within a temperature range of 4-32 °C. The pH growth tolerance ranges between pH 5 and 10, and it tolerates 4% (w/v) NaCl. A 16S rRNA phylogenetic analysis positioned strain FIT81 within the genus , and multilocus sequence analysis revealed that IzPS32d, MS586, IzPS23, '' 46-2 and PS9-14 are the top five most closely related species, which were selected for further genome-to-genome comparisons, as well as for physiological and chemotaxonomic characterization. The genome size of strain FIT81 is 6 492 796 base-pairs long, with 60.6 mol% of G+C content. Average nucleotide identity and digital DNA-DNA hybridization analyses yielded values of 93.6 and 56.1%, respectively, when the FIT81 genome was compared to that of the closest type strain IzPS32d. Taken together, the obtained genomic, physiologic and chemotaxonomic data indicate that strain FIT81 is different from its closest relative species, which lead us to suggest that it is a novel species to be included in the list of type strains with the name sp. nov. (FIT81=CECT 30374=DSM 112699).
在名为 fitomatics 的研究项目框架内，我们从 的根茎中分离并鉴定了一种细菌植物内生菌，以下简称菌株 FIT81。该细菌革兰氏阴性，杆状，具端生丛毛状鞭毛，过氧化氢酶和氧化酶阳性。菌株 FIT81 的最适生长温度为 28°C，但可在 4-32°C 的温度范围内生长。其 pH 生长耐受范围在 pH 5 和 10 之间，可耐受 4%（w/v）NaCl。16S rRNA 系统发育分析将菌株 FIT81 定位于 属内，多位点序列分析显示， IzPS32d、MS586、IzPS23、46-2 和 PS9-14 是与菌株 FIT81 关系最密切的五个种，选择它们进行进一步的基因组到基因组比较，以及生理和化学分类学特征分析。菌株 FIT81 的基因组大小为 6492796 碱基对，G+C 含量为 60.6 mol%。当将 FIT81 基因组与最接近的模式菌株 IzPS32d 进行比较时，平均核苷酸同一性和数字 DNA-DNA 杂交分析分别产生了 93.6%和 56.1%的值。综合获得的基因组、生理和化学分类学数据表明，菌株 FIT81 与其最接近的相关种不同，这导致我们建议将其作为一个新种列入模式菌株名单，命名为 sp. nov.（FIT81=CECT 30374=DSM 112699）。
