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环状 RNA hsa_circ_0003823 通过 miR-607/CRISP3 轴促进食管鳞癌的肿瘤进展、转移和阿帕替尼耐药。

Circular RNA hsa_circ_0003823 promotes the Tumor Progression, Metastasis and Apatinib Resistance of Esophageal Squamous Cell Carcinoma by miR-607/CRISP3 Axis.

机构信息

Department of Thoracic Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, P.R. China.

Department of General Surgery, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200000, P.R. China.

出版信息

Int J Biol Sci. 2022 Sep 21;18(15):5787-5808. doi: 10.7150/ijbs.76096. eCollection 2022.

DOI:10.7150/ijbs.76096
PMID:36263172
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9576509/
Abstract

Circular RNAs (CircRNAs) have attracted a growing interest of research in cancer. The regulatory roles and mechanisms of circRNAs in progression, metastasis and drug resistance of esophageal squamous cell carcinoma (ESCC) needed to be clarified. Our previous study revealed the crucial role of Apatinib in ESCC therapy. However, the correlation between circRNAs and Apatinib resistance remained unclear. 3 pairs of tumor and paracancerous tissues of ESCC patients were used for RNA sequencing. Western blot analysis, RNA immunoprecipitation (RIP), dual-luciferase reporter assays, apoptosis and animal assays were conducted to confirm the roles and specific mechanisms of hsa_circ_0003823 as well as the effects of it on Apatinib sensitivity in ESCC. Our results revealed that hsa_circ_0003823 was highly expressed in ESCC and associated with poor prognosis. Further results indicated that hsa_circ_0003823 promoted proliferation and metastasis ability of ESCC. In the section of mechanism experiments, hsa_circ_0003823 regulated CRISP3 by targeting microRNA-607 (miR-607) to promote progression of ESCC. Besides, we found that silencing hsa_circ_0003823 improved Apatinib sensitivity. hsa_circ_0003823 resulted in Apatinib resistance by miR-607/CRISP3 axis. In this study, we elucidated the function of hsa_circ_0003823 and its role in promoting tumor progression, metastasis and Apatinib resistance of ESCC through miR-607/CRISP3 axis.

摘要

环状 RNA（CircRNAs）在癌症研究中引起了越来越多的关注。环状 RNA 在食管鳞状细胞癌（ESCC）进展、转移和耐药中的调控作用和机制需要阐明。我们之前的研究表明阿帕替尼在 ESCC 治疗中的重要作用。然而，环状 RNA 与阿帕替尼耐药之间的相关性尚不清楚。

使用 3 对 ESCC 患者的肿瘤和癌旁组织进行 RNA 测序。通过 Western blot 分析、RNA 免疫沉淀（RIP）、双荧光素酶报告基因测定、凋亡和动物实验，证实了 hsa_circ_0003823 的作用及其对 ESCC 中阿帕替尼敏感性的具体机制，以及其对阿帕替尼敏感性的影响。

我们的结果表明，hsa_circ_0003823 在 ESCC 中高表达，与预后不良相关。进一步的结果表明，hsa_circ_0003823 促进了 ESCC 的增殖和转移能力。在机制实验部分，hsa_circ_0003823 通过靶向 microRNA-607（miR-607）来调节 CRISP3，从而促进 ESCC 的进展。此外，我们发现沉默 hsa_circ_0003823 可以提高阿帕替尼的敏感性。hsa_circ_0003823 通过 miR-607/CRISP3 轴导致阿帕替尼耐药。

在这项研究中，我们通过 miR-607/CRISP3 轴阐明了 hsa_circ_0003823 的功能及其在促进 ESCC 肿瘤进展、转移和阿帕替尼耐药中的作用。

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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba0a/9576509/c0d11d8aa367/ijbsv18p5787g012.jpg

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本文引用的文献

1
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2
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Cell Mol Life Sci. 2022 Feb 3;79(2):116. doi: 10.1007/s00018-022-04131-6.
3
The emerging roles of circRNAs in cancer and oncology.环状RNA在癌症和肿瘤学中的新兴作用。
Cell Mol Life Sci. 2025 Apr 24;82(1):174. doi: 10.1007/s00018-025-05651-7.
4
circ-TTC17 Promotes Esophagus Squamous Cell Carcinoma Cell Growth, Metastasis, and Inhibits Autophagy-Mediated Radiosensitivity Through miR-145-5p/SIRT1 Axis.环状TTC17通过miR-145-5p/SIRT1轴促进食管鳞状细胞癌细胞生长、转移并抑制自噬介导的放射敏感性。
Thorac Cancer. 2025 Jan;16(1):e15494. doi: 10.1111/1759-7714.15494. Epub 2024 Dec 2.
5
CAP superfamily proteins in human: a new target for cancer therapy.人类 CAP 超家族蛋白：癌症治疗的新靶点。
Med Oncol. 2024 Nov 5;41(12):306. doi: 10.1007/s12032-024-02548-6.
6
Emerging roles of non-coding RNAs in modulating the PI3K/Akt pathway in cancer.非编码RNA在调控癌症中PI3K/Akt信号通路方面的新作用
Noncoding RNA Res. 2024 Aug 9;10:1-15. doi: 10.1016/j.ncrna.2024.08.002. eCollection 2025 Feb.
7
Computational insights into CRISP3 downregulation in cervical cancer and its cervical lineages pattern.宫颈癌中CRISP3下调及其宫颈谱系模式的计算洞察。
Precis Clin Med. 2024 Jul 24;7(3):pbae016. doi: 10.1093/pcmedi/pbae016. eCollection 2024 Sep.
8
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Nat Rev Clin Oncol. 2022 Mar;19(3):188-206. doi: 10.1038/s41571-021-00585-y. Epub 2021 Dec 15.
4
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Acta Pharm Sin B. 2021 Nov;11(11):3379-3392. doi: 10.1016/j.apsb.2021.03.008. Epub 2021 Mar 9.
5
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J Exp Clin Cancer Res. 2021 Nov 15;40(1):361. doi: 10.1186/s13046-021-02149-5.
6
Phosphoproteomics reveals therapeutic targets of esophageal squamous cell carcinoma.磷酸化蛋白质组学揭示食管鳞状细胞癌的治疗靶点。
Signal Transduct Target Ther. 2021 Nov 12;6(1):381. doi: 10.1038/s41392-021-00682-5.
7
Interaction with WTAP Promotes Assembly and Activity of the mA Methyltransferase Complex and Promotes Cisplatin Resistance in Bladder Cancer.WTAP 相互作用促进 mA 甲基转移酶复合物的组装和活性，并促进膀胱癌的顺铂耐药性。
Cancer Res. 2021 Dec 15;81(24):6142-6156. doi: 10.1158/0008-5472.CAN-21-1518. Epub 2021 Oct 26.
8
Advances in the curative management of oesophageal cancer.食管癌治疗管理的进展。
Br J Cancer. 2022 Mar;126(5):706-717. doi: 10.1038/s41416-021-01485-9. Epub 2021 Oct 21.
9
Circular RNA circSNX6 promotes sunitinib resistance in renal cell carcinoma through the miR-1184/GPCPD1/ lysophosphatidic acid axis.环状 RNA circSNX6 通过 miR-1184/GPCPD1/溶血磷脂酸轴促进肾细胞癌对舒尼替尼的耐药性。
Cancer Lett. 2021 Dec 28;523:121-134. doi: 10.1016/j.canlet.2021.10.003. Epub 2021 Oct 7.
10
Role of Adjuvant Therapy in Esophageal Cancer Patients After Neoadjuvant Therapy and Esophagectomy: A Systematic Review and Meta-analysis.新辅助治疗和食管切除术后辅助治疗在食管癌患者中的作用：系统评价和荟萃分析。
Ann Surg. 2022 Jan 1;275(1):91-98. doi: 10.1097/SLA.0000000000005227.