Petrosius Valdemaras, Schoof Erwin M
Department of Biotechnology and Biomedicine, Technical University of Denmark, Lyngby, Denmark.
Department of Biotechnology and Biomedicine, Technical University of Denmark, Lyngby, Denmark.
Transl Oncol. 2023 Jan;27:101556. doi: 10.1016/j.tranon.2022.101556. Epub 2022 Oct 19.
The field of single-cell omics is rapidly progressing. Although DNA and RNA sequencing-based methods have dominated the field to date, global proteome profiling has also entered the main stage. Single-cell proteomics was facilitated by advancements in different aspects of mass spectrometry (MS)-based proteomics, such as instrument design, sample preparation, chromatography and ion mobility. Single-cell proteomics by mass spectrometry (scp-MS) has moved beyond being a mere technical development, and is now able to deliver actual biological application and has been successfully applied to characterize different cell states. Here, we review some key developments of scp-MS, provide a background to the field, discuss the various available methods and foresee possible future directions.
单细胞组学领域正在迅速发展。尽管基于DNA和RNA测序的方法至今在该领域占据主导地位，但全球蛋白质组分析也已进入主要阶段。基于质谱（MS）的蛋白质组学在仪器设计、样品制备、色谱和离子淌度等不同方面的进展推动了单细胞蛋白质组学的发展。基于质谱的单细胞蛋白质组学（scp-MS）已不仅仅是一项技术发展，现在能够实现实际的生物学应用，并已成功应用于表征不同的细胞状态。在此，我们回顾scp-MS的一些关键进展，提供该领域的背景信息，讨论各种可用方法，并展望未来可能的发展方向。
