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Effects of mutations in the TATA box region of the Agrobacterium T-cyt gene on its transcription in plant tissues.

作者信息

de Pater B S, de Kam R J, Hoge J H, Schilperoort R A

机构信息

Molbas Research Group, Department of Plant Molecular Biology, Leiden University, The Netherlands.

出版信息

Nucleic Acids Res. 1987 Oct 26;15(20):8283-92. doi: 10.1093/nar/15.20.8283.

DOI:10.1093/nar/15.20.8283
PMID:3671084
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC306359/
Abstract

We have generated mutations in the promoter region of the octopine type cytokinin gene of Agrobacterium tumefaciens, and studied their effects on mRNA formation in different plant species. The promoter region of this gene contains several putative TATA boxes. Phenotypic expression and Northern blot hybridization showed that TATA boxes are essential for expression, but that one TATA box leads to wild-type transcript levels. Analysis of the 5' ends of T-cyt transcripts by primer extension using RNA from T-cyt gene transformed tobacco shoots revealed two major cap site clusters and one minor cap site. TATA box consensus sequences can be found approximately 30 bp upstream from each cap site cluster. Deletion of a TATA box results in loss of the corresponding cap sites. An insertion of 7 bp between the right TATA box and corresponding cap sites results in a shift of the position of the cap sites, so that the original distance of TATA box to cap sites is conserved as much as possible.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bfa/306359/73b76376fd91/nar00264-0172-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bfa/306359/31e5366250d9/nar00264-0171-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bfa/306359/73b76376fd91/nar00264-0172-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bfa/306359/31e5366250d9/nar00264-0171-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bfa/306359/73b76376fd91/nar00264-0172-a.jpg

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本文引用的文献

1
Tumour genes in plants: T-DNA encoded cytokinin biosynthesis.植物中的肿瘤基因：T-DNA编码的细胞分裂素生物合成。
EMBO J. 1985 Apr;4(4):853-9. doi: 10.1002/j.1460-2075.1985.tb03710.x.
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Organization and expression of eucaryotic split genes coding for proteins.编码蛋白质的真核生物断裂基因的组织与表达。
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Nucleotide sequence and transcript mapping of the tmr gene of the pTiA6NC octopine Ti-plasmid: a bacterial gene involved in plant tumorigenesis.章鱼碱型Ti质粒pTiA6NC的tmr基因的核苷酸序列及转录图谱：一个参与植物肿瘤发生的细菌基因
根癌农杆菌pTiA6 ipt基因启动子在转基因烟草中的表达水平及表达位置
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5
Regulation of Agrobacterium tumefaciens T-cyt gene expression in leaves of transgenic potato (Solanum tuberosum L. cv. Désirée) is strongly influenced by plant culture conditions.根癌农杆菌T-细胞基因在转基因马铃薯（Solanum tuberosum L. cv. Désirée）叶片中的表达调控受到植物培养条件的强烈影响。
Plant Mol Biol. 1991 Oct;17(4):711-25. doi: 10.1007/BF00037056.
J Mol Appl Genet. 1984;2(4):354-62.
4
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Nucleic Acids Res. 1983 Sep 24;11(18):6211-23. doi: 10.1093/nar/11.18.6211.
5
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6
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