El-Samad Lamia M, Hassan Mohamed A, Basha Amal A, El-Ashram Saeed, Radwan Eman H, Abdul Aziz Karoline K, Tamer Tamer M, Augustyniak Maria, El Wakil Abeer
Department of Zoology, Faculty of Science, Alexandria University, Alexandria, Egypt.
Protein Research Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, P.O. Box: 21934, Alexandria, Egypt; University Medical Center Göttingen, Georg-August-University, 37073 Göttingen, Germany.
Int J Pharm. 2022 Dec 15;629:122328. doi: 10.1016/j.ijpharm.2022.122328. Epub 2022 Oct 22.
Diabetic wound healing encounters significant challenges due to the extreme oxidative stress resulting from excessive inflammation and microbial infections, disrupting the typical cascade of wound healing and thwarting the re-epithelialization of skin tissues. Benefiting from the biological activities of carboxymethyl cellulose (CMC) and sericin, we thus fabricated multifunctional hydrogels of CMC-Sericin. The hydrogel revealed high swelling performance alongside its porous structure. The incorporation of sericin bestowed the CMC-Sericin hydrogels with a prominent capacity to scavenge free radicals and antibacterial activity. In vivo investigations using diabetic full-thickness excision wounds demonstrated the capability of CMC-Sericin dressing to enhance diabetic wounds in rats treated or untreated with insulin concurrently. Furthermore, histopathological examinations manifested the skin tissue regeneration evidenced by the development of skin appendages like hair follicles and collagen deposition after treatment with CMC-Sericin hydrogel. Moreover, the levels of antioxidant parameters, including GSH and SOD, were substantially augmented and associated with a significant diminution in lipid peroxidation, implying a decrease in oxidative stress in the tissues. Beyond that, CMC-Sericin dressing downregulated the pro-inflammatory markers and upregulated the heat shock proteins, indicating the restoration of physiological features in cells. Strikingly, CMC-Sericin dressing remarkably promoted the healing of diabetic wounds without insulin treatment.
糖尿病伤口愈合面临重大挑战，因为过度炎症和微生物感染会导致极端的氧化应激，破坏典型的伤口愈合级联反应，阻碍皮肤组织的重新上皮化。受益于羧甲基纤维素（CMC）和丝胶蛋白的生物活性，我们制备了CMC-丝胶蛋白多功能水凝胶。该水凝胶具有高膨胀性能及其多孔结构。丝胶蛋白的加入赋予了CMC-丝胶蛋白水凝胶显著的自由基清除能力和抗菌活性。使用糖尿病全层切除伤口进行的体内研究表明，CMC-丝胶蛋白敷料能够增强同时接受或未接受胰岛素治疗的大鼠的糖尿病伤口愈合。此外，组织病理学检查显示，在用CMC-丝胶蛋白水凝胶治疗后，皮肤附属器如毛囊的发育和胶原蛋白沉积证明了皮肤组织的再生。此外，抗氧化参数（包括谷胱甘肽和超氧化物歧化酶）水平显著提高，同时脂质过氧化显著减少，这意味着组织中的氧化应激降低。除此之外，CMC-丝胶蛋白敷料下调促炎标志物并上调热休克蛋白，表明细胞生理特征得以恢复。令人惊讶的是，CMC-丝胶蛋白敷料在未进行胰岛素治疗的情况下显著促进了糖尿病伤口的愈合。
