Baptista-Silva Sara, Bernardes Beatriz G, Borges Sandra, Rodrigues Ilda, Fernandes Rui, Gomes-Guerreiro Susana, Pinto Marta Teixeira, Pintado Manuela, Soares Raquel, Costa Raquel, Oliveira Ana Leite
CBQF-Centro de Biotecnologia e Química Fina, Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, 4169-005 Porto, Portugal.
I+D Farma Group (GI-1645), Department of Pharmacology, Pharmacy and Pharmaceutical Technology, iMATUS and Health Research Institute of Santiago de Compostela (IDIS), Universidade de Santiago de Compostela, E-15782 Santiago de Compostela, Spain.
Biomolecules. 2022 Jun 8;12(6):801. doi: 10.3390/biom12060801.
Chronic wounds are one of the most frequent complications that are associated with diabetes mellitus. The overproduction of reactive oxygen species (ROS) is a key factor in the delayed healing of a chronic wound. In the present work, we develop a novel in situ-forming silk sericin-based hydrogel (SSH) that is produced by a simple methodology using horseradish peroxidase (HRP) crosslinking as an advanced dressing for wound healing. The antioxidant and angiogenic effects were assessed in vitro and in vivo after in situ application using an excisional wound-healing model in a genetically-induced diabetic db/db mice and though the chick embryo choriollantoic membrane (CAM) assay, respectively. Wounds in diabetic db/db mice that were treated with SSH closed with reduced granulation tissue, decreased wound edge distance, and wound thickness, when compared to Tegaderm, a dressing that is commonly used in the clinic. The hydrogel also promoted a deposition of collagen fibers with smaller diameter which may have had a boost effect in re-epithelialization. SSH treatment slightly induced two important endogenous antioxidant defenses, superoxide dismutase and catalase. A CAM assay made it possible to observe that SSH led to an increase in the number of newly formed vessels without inducing an inflammatory reaction. The present hydrogel may result in a multi-purpose technology with angiogenic, antioxidant, and anti-inflammatory properties, while advancing efficient and organized tissue regeneration.
慢性伤口是糖尿病最常见的并发症之一。活性氧（ROS）的过度产生是慢性伤口愈合延迟的关键因素。在本研究中，我们开发了一种新型的原位形成的丝胶基水凝胶（SSH），它通过一种简单的方法制备，使用辣根过氧化物酶（HRP）交联，作为伤口愈合的高级敷料。分别使用基因诱导的糖尿病db/db小鼠的切除伤口愈合模型和鸡胚绒毛尿囊膜（CAM）试验，在原位应用后在体外和体内评估其抗氧化和血管生成作用。与临床常用的敷料Tegaderm相比，用SSH治疗的糖尿病db/db小鼠伤口愈合时肉芽组织减少，伤口边缘距离和伤口厚度降低。水凝胶还促进了直径较小的胶原纤维的沉积，这可能对再上皮化有促进作用。SSH处理轻微诱导了两种重要的内源性抗氧化防御，即超氧化物歧化酶和过氧化氢酶。CAM试验表明，SSH可增加新形成血管的数量，且不诱导炎症反应。目前的水凝胶可能会产生一种具有血管生成、抗氧化和抗炎特性的多功能技术，同时促进高效和有组织的组织再生。
