Microbiology Department, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq.
Zoonosis Research Unit, Department of Veterinary Public Health, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq.
Arch Razi Inst. 2022 Apr 30;77(2):697-701. doi: 10.22092/ARI.2022.356982.1955. eCollection 2022 Apr.
Plants have been long valuable sources of natural materials that have served to preserve human and animal health; as a result, pharmacological purposes have arisen from the use of plant compounds in most countries, according to a World Health Organization report. The present study aimed to assess the antimicrobial resistance of tannin extract against () isolates in sheep. A total of 100 samples from sheep were used to isolate and treated with tannin extract (90% purity) to investigate the effect, as compared to some antibiotics (Clindamycin, Cephalexin, Kanamycin, Tetracycline, and Vancomycin). The bacterial samples were cultured in a selective and differential medium, and Gram staining was used to examine them. The biochemical assays were performed to purify and expose these cultures; moreover, the API 20E system and Rapid ONE kits were utilized to confirm the bacterial strain. Based on the findings, 50% of the samples showed a positive result for the presence of . The well diffusion technique was used to investigate the antibacterial activity to confirm the antibacterial action of tannin extract (from pomegranate peel) in different concentrations against . The highest zone of inhibition for the bacteria ranged from 12±0.5 to 30.3±0.2 at 50% concentrations, proving that tannins extract was significantly effective against . The presence of was detected in 50 % of the samples. The well-diffusion technique was used to evaluate the antimicrobial property of tannin extract through various concentrations with the highest zone of inhibition for the bacteria ranging from 12.5 to 30.30.2 at 50%, demonstrating that tannin extract was significantly effective on .
植物一直是天然材料的重要来源，这些材料有助于维护人类和动物的健康；因此，根据世界卫生组织的一份报告，大多数国家都将植物化合物用于药理学目的。本研究旨在评估单宁提取物对绵羊中 () 分离株的抗菌耐药性。从绵羊中采集了 100 个样本，以分离 和用单宁提取物（纯度为 90%）进行处理，以研究与某些抗生素（克林霉素、头孢氨苄、卡那霉素、四环素和万古霉素）相比的 效果。将细菌样本在选择性和鉴别培养基中培养，并使用革兰氏染色法对其进行检查。进行生化分析以纯化和暴露这些培养物；此外，还使用 API 20E 系统和 Rapid ONE 试剂盒来确认细菌株。根据研究结果，50%的样本显示出存在 的阳性结果。采用打孔扩散技术来研究抗菌活性，以确认单宁提取物（来自石榴皮）在不同浓度下对 的抗菌作用。细菌的最高抑菌环直径范围为 50%浓度下的 12±0.5 至 30.3±0.2，证明单宁提取物对 具有显著的抑制作用。在 50%的样本中检测到 的存在。采用打孔扩散技术评估单宁提取物的抗菌性能，通过不同浓度进行评估，细菌的最高抑菌环直径范围为 50%浓度下的 12.5 至 30.30.2，表明单宁提取物对 具有显著的抑制作用。
