Maleki Dizaj Solmaz, Shokrgozar Hojjat, Yazdani Javad, Memar Mohammad Yousef, Sharifi Simin, Ghavimi Mohammad Ali
Dental and Periodontal Research Center, Tabriz University of Medical Sciences, Tabriz 5166, Iran.
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz 5166, Iran.
Clin Pract. 2022 Oct 6;12(5):809-817. doi: 10.3390/clinpract12050085.
Background. Despite their benefits, dental implants may sometimes fail for a diversity of causes; the most common reasons of failure are infection and bone loss. Porphyromonas gingivalis (P. gingivalis) bacteria show a major role in peri-implantitis infection and dental implant failure. Methods. In this study, the prevalence of P. gingivalis isolated from the gingival crevicular fluid (GCF) of fifteen Iranian patients with implant failure (more than 1/3 of the implant length), who had average oral and dental hygiene and no antibiotic use for at least one month, was determined. Moreover, the antimicrobial effects of curcumin nanocrystals against isolated P. gingivalis were investigated. The collected samples from patients were transferred to a microbiology laboratory to culture. The presence of P. gingivalis in the culture media was confirmed using a trypsin reagent test. An isolate from a patient with the highest colony count was selected to evaluate the antibacterial effects of curcumin nanoparticles. The inhibition zone diameter, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) were determined. Results. Out of fifteen patients, eight (53.33%) were positive for the presence of P. gingivalis. The results of the microbial tests showed that curcumin nanoparticles had an MIC of 6.25 µg/mL and an MBC of 12.5 µg/mL. Conclusions. The use of curcumin nanoparticles may control the bacterial infection around the implant.
背景。尽管牙种植体有诸多益处，但有时可能因多种原因而失败；最常见的失败原因是感染和骨质流失。牙龈卟啉单胞菌（牙龈卟啉单胞菌）在种植体周围炎感染和牙种植体失败中起主要作用。方法。在本研究中，测定了从15名伊朗种植体失败患者（种植体长度超过1/3）的龈沟液（GCF）中分离出的牙龈卟啉单胞菌的患病率，这些患者口腔和牙齿卫生状况一般，且至少一个月未使用抗生素。此外，还研究了姜黄素纳米晶体对分离出的牙龈卟啉单胞菌的抗菌作用。从患者收集的样本被转移到微生物实验室进行培养。使用胰蛋白酶试剂试验确认培养基中是否存在牙龈卟啉单胞菌。从菌落计数最高的患者中分离出一株菌，以评估姜黄素纳米颗粒的抗菌效果。测定抑菌圈直径、最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。结果。15名患者中，8名（53.33%）牙龈卟啉单胞菌检测呈阳性。微生物试验结果表明，姜黄素纳米颗粒的MIC为6.25μg/mL，MBC为12.5μg/mL。结论。姜黄素纳米颗粒的使用可能控制种植体周围的细菌感染。
