Yang Chul Min, Lee Jeehee, Lee Su Yeon, Lee Haeshin, Chathuranga Kiramage, Lee Jongsoo, Park Wonho
Department of Organic Materials Engineering, Chungnam National University, Daejeon 34134, Korea.
Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Korea.
Gels. 2022 Oct 12;8(10):650. doi: 10.3390/gels8100650.
The inevitable bleeding and infections caused by disasters and accidents are the main causes of death owing to extrinsic trauma. Hemostatic agents are often used to quickly suppress bleeding and infection, and they can solve this problem in a short time. Silk fibroin (SF) has poor processibility in water, owing to incomplete solubility therein. In this study, aiming to overcome this disadvantage, a modified silk fibroin (SF-BGE), easily soluble in water, was prepared by introducing butyl glycidyl ether (BGE) into its side chain. Subsequently, a small amount of tannic acid (TA) was introduced to prepare an SF-BGE /TA solution, and ZnO nanoparticles (NPs) were added to the solution to form the coordination bonds between the ZnO and TA, leading to an SF-based nanocomposite hydrogel. A structural characterization of the SF-BGE, SF-BGE/TA, SF-BGE/TA/ZnO, and the coordination bonds between ZnO/TA was observed by attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), and the phase change was observed by rheological measurements. The pore formation of the SF-BGE/TA/ZnO hydrogel and dispersibility of ZnO were verified through energy-dispersive X-ray spectroscopy (EDS) and scanning electron microscopy (SEM). The cytocompatible and hemostatic performances of the SF-BGE/TA/ZnO NPs composite hydrogels were evaluated, and the hydrogels showed superior hemostatic and cytocompatible activities. Therefore, the SF-based nanocomposite hydrogel is considered as a promising material for hemostasis.
由灾难和事故导致的不可避免的出血和感染是外部创伤致死的主要原因。止血剂常被用于快速抑制出血和感染，并且它们能在短时间内解决这个问题。丝素蛋白（SF）由于在水中不完全溶解，其加工性能较差。在本研究中，为了克服这一缺点，通过将丁基缩水甘油醚（BGE）引入其侧链制备了一种易溶于水的改性丝素蛋白（SF-BGE）。随后，引入少量单宁酸（TA）以制备SF-BGE/TA溶液，并向该溶液中添加氧化锌纳米颗粒（NPs）以形成氧化锌与TA之间的配位键，从而得到一种基于丝素蛋白的纳米复合水凝胶。通过衰减全反射傅里叶变换红外光谱（ATR-FTIR）观察了SF-BGE、SF-BGE/TA、SF-BGE/TA/ZnO的结构特征以及ZnO/TA之间的配位键，并通过流变学测量观察了相变。通过能量色散X射线光谱（EDS）和扫描电子显微镜（SEM）验证了SF-BGE/TA/ZnO水凝胶的孔隙形成和ZnO的分散性。评估了SF-BGE/TA/ZnO NPs复合水凝胶的细胞相容性和止血性能，该水凝胶表现出优异的止血和细胞相容活性。因此，基于丝素蛋白的纳米复合水凝胶被认为是一种有前景的止血材料。
