Institute for Insect Biotechnology, Justus-Liebig-University Giessen, 35392 Giessen, Germany.
Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Branch for Bioresources, 35392 Giessen, Germany.
Mar Drugs. 2022 Sep 30;20(10):620. doi: 10.3390/md20100620.
Marine flavobacterium Tenacibaculum discolor sv11 has been proven to be a promising producer of bioactive nitrogen-containing heterocycles. A chemical investigation of T. discolor sv11 revealed seven new heterocycles, including the six new imidazolium-containing alkaloids discolins C-H (1−6) and one pyridinium-containing alkaloid dispyridine A (7). The molecular structure of each compound was elucidated by analysis of NMR and HR-ESI-MS data. Furthermore, enzymatic decarboxylation of tryptophan and tyrosine to tryptamine and tyramine catalyzed by the decarboxylase DisA was investigated using in vivo and in vitro experiments. The antimicrobial activity of the isolated compounds (1−7) was evaluated. Discolin C and E (1 and 3) exhibited moderate activity against Gram-positive Bacillus subtilis DSM10, Mycobacterium smegmatis ATCC607, Listeria monocytogenes DSM20600 and Staphylococcus aureus ATCC25923, with MIC values ranging from 4 μg/mL to 32 μg/mL.
海洋黄杆菌 Tenacibaculum discolor sv11 已被证明是一种有前途的生物活性含氮杂环化合物的生产者。对 T. discolor sv11 的化学研究揭示了七种新的杂环化合物，包括六种新的含咪唑啉的生物碱 discolins C-H（1-6）和一种含吡啶的生物碱 dispyridine A（7）。通过分析 NMR 和 HR-ESI-MS 数据确定了每个化合物的分子结构。此外，使用体内和体外实验研究了脱羧酶 DisA 催化色氨酸和酪氨酸脱羧生成色胺和酪胺的反应。评估了分离得到的化合物（1-7）的抗菌活性。Discolin C 和 E（1 和 3）对革兰氏阳性枯草芽孢杆菌 DSM10、耻垢分枝杆菌 ATCC607、单核细胞增生李斯特菌 DSM20600 和金黄色葡萄球菌 ATCC25923 表现出中等活性，MIC 值范围为 4 μg/mL 至 32 μg/mL。
