Mosetti Veronica, Rosetti Beatrice, Pierri Giovanni, Bellotto Ottavia, Adorinni Simone, Bandiera Antonella, Adami Gianpiero, Tedesco Consiglia, Crosera Matteo, Magnano Greta Camilla, Marchesan Silvia
Chemical and Pharmaceutical Sciences Department, University of Trieste, 34127 Trieste, Italy.
Department of Chemistry and Biology "A. Zambelli", University of Salerno, 84084 Fisciano, Italy.
Biomedicines. 2022 Sep 20;10(10):2342. doi: 10.3390/biomedicines10102342.
Cyclodipeptides (CDPs) or diketopiperazines (DKPs) are often found in nature and in foodstuff and beverages and have attracted great interest for their bioactivities, biocompatibility, and biodegradability. In the laboratory, they can be prepared by green procedures, such as microwave-assisted cyclization of linear dipeptides in water, as performed in this study. In particular, five CDPs were prepared and characterized by a variety of methods, including NMR and ESI-MS spectroscopies and single-crystal X-ray diffraction (XRD), and their cytocompatibility and anti-aging activity was tested in vitro, as well as their ability to penetrate the different layers of the skin. Although their mechanism of action remains to be elucidated, this proof-of-concept study lays the basis for their future use in anti-age cosmetic applications.
环二肽（CDPs）或二酮哌嗪（DKPs）在自然界以及食品和饮料中经常被发现，因其生物活性、生物相容性和生物降解性而备受关注。在实验室中，它们可以通过绿色方法制备，比如本研究中所进行的在水中微波辅助线性二肽环化反应。具体而言，制备了五种环二肽，并通过多种方法进行了表征，包括核磁共振（NMR）和电喷雾电离质谱（ESI-MS）光谱分析以及单晶X射线衍射（XRD），还在体外测试了它们的细胞相容性和抗衰老活性，以及它们穿透皮肤不同层的能力。尽管其作用机制仍有待阐明，但这项概念验证研究为其未来在抗老化化妆品应用中的使用奠定了基础。
