Li Ruichao, Zhang Lifei, Lu Xiaoyu, Peng Kai, Liu Yuan, Xiao Xia, Song Hongqin, Wang Zhiqiang
Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.
Institute of Comparative Medicine, Yangzhou University, Yangzhou 225009, China.
Antibiotics (Basel). 2022 Oct 17;11(10):1422. doi: 10.3390/antibiotics11101422.
Bacteria with carbapenem or tigecycline resistance have been spreading widely among humans, animals and the environment globally, being great threats to public health. However, bacteria co-carrying drug resistance genes of carbapenem and tigecycline in and species remain to be investigated. Here, we detected nine -carrying spp. isolates as well as three isolates co-harboring (X3) and from seventy-two samples collected from a dairy farm in China. To explore their genomic characteristic and transmission mechanism, we utilized various methods, including PCR, antimicrobial susceptibility testing, conjugation experiment, whole-genome sequencing, circular intermediate identification and bioinformatics analysis. Clonal dissemination was found among three , of which (X3) and were located on a novel non-conjugative plasmid pJNE5-X3_NDM-1 (333,311 bp), and the circular intermediate ΔIS-(X3)- was identified. Moreover, there was another copy of (X3) on the chromosome of . It was verified that could be transferred to C600 from spp. by conjugation, and self-transmissible IncA/C plasmids mediated the transmission of in spp. strains. Stringent surveillance was warranted to curb the transmission of such vital resistance genes.
对碳青霉烯类或替加环素耐药的细菌已在全球范围内的人类、动物和环境中广泛传播，对公众健康构成巨大威胁。然而，同时携带碳青霉烯类和替加环素耐药基因的细菌在[具体物种1]和[具体物种2]中仍有待研究。在此，我们从中国一家奶牛场采集的72份样本中检测到9株携带[耐药基因1]的[物种1]分离株以及3株同时携带[耐药基因2]（X3）和[耐药基因3]的[物种2]分离株。为探究它们的基因组特征和传播机制，我们采用了多种方法，包括聚合酶链反应（PCR）、药敏试验、接合试验、全基因组测序、环状中间体鉴定和生物信息学分析。在3株[物种1]之间发现了克隆传播，其中[耐药基因2]（X3）和[耐药基因3]位于一个新型非接合性质粒pJNE5-X3_NDM-1（333,311 bp）上，并鉴定出环状中间体ΔIS-[耐药基因2]-(X3)-[耐药基因3]。此外，在[物种1]的染色体上还有另一个[耐药基因2]（X3）拷贝。经证实，[耐药基因2]可通过接合作用从[物种1]转移至大肠埃希菌C600，并且自我传递的IncA/C质粒介导了[耐药基因2]在[物种1]菌株中的传播。有必要进行严格监测以遏制此类重要耐药基因的传播。
