Elsayed Mahmoud E, Abd El-Hamid Marwa I, El-Gedawy Attia, Bendary Mahmoud M, ELTarabili Reham M, Alhomrani Majid, Alamri Abdulhakeem S, Alghamdi Saleh A, Arnout Marwa, Binjawhar Dalal N, Al-Sanea Mohammad M, Abousaty Amira I
Department of Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt.
Department of Microbiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt.
Antibiotics (Basel). 2022 Oct 21;11(10):1447. doi: 10.3390/antibiotics11101447.
Listeriosis is one of the most common foodborne diseases caused by (). A poor prognosis has been recorded for the invasive listeriosis, especially neurolisteriosis. In several countries throughout the world, foodborne infections with exceeded the legal safety limits in animal sourced foods. Therefore, we decided to investigate the variability, virulence and antimicrobial resistance profiles of this pathogen. Both phenotypic and genotypic methods were used for identifying isolates and confirming their virulence profiles. The antimicrobial resistances and their correlation analysis with the existence of virulence genes were detected. Additionally, sequencing and phylogenetic analysis based on and genes were undertaken. The prevalence rate (11.9%) and the resistance profiles of were shocking. The multi-drug resistance (MDR) phenotypes were common among our isolates (64.9%). Fortunately, the resistance phenotypes were always associated with low virulence arrays and the MDR strains possessed low virulence fitness. Herein, the high genotypic and phenotypic diversity of isolates and their weak clonality and adaptability highlighted the difficulty in controlling and managing this pathogen. Therefore, it is important to add more restriction guidelines from national authorities on the consumption of ready to eat foods.
李斯特菌病是由（）引起的最常见的食源性疾病之一。侵袭性李斯特菌病，尤其是神经型李斯特菌病，预后较差。在世界上几个国家，动物源食品中食源性感染（）的情况超过了法定安全限度。因此，我们决定调查这种病原体的变异性、毒力和抗菌药物耐药性特征。采用表型和基因型方法鉴定（）分离株并确认其毒力特征。检测了抗菌药物耐药性及其与毒力基因存在情况的相关性分析。此外，还基于（）和（）基因进行了测序和系统发育分析。（）的流行率（11.9%）和耐药特征令人震惊。多重耐药（MDR）表型在我们的分离株中很常见（64.9%）。幸运的是，耐药表型总是与低毒力组合相关，并且多重耐药菌株的毒力适应性较低。在此，（）分离株的高基因型和表型多样性及其较弱的克隆性和适应性凸显了控制和管理这种病原体的困难。因此，国家当局增加对即食食品消费的更多限制指南很重要。
