Robinson Kelsy, Assumpcao Anna L F V, Arsi Komala, Erf Gisela F, Donoghue Annie, Jesudhasan Palmy R R
Poultry Research Unit, ARS, USDA, Mississippi State, MS 39762, USA.
Department of Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA.
Animals (Basel). 2022 Oct 20;12(20):2867. doi: 10.3390/ani12202867.
Reducing in commercial chickens is vital to decreasing human salmonellosis infections resulting from contact with contaminated poultry and poultry products. As the intestinal microbiota plays an important role in preventing pathogen colonization, we sought to understand the relationship between infection and the cecal microbiota and the host immune system. Day-of-hatch broiler chicks were assigned to three treatments: control, artificial (SA), and natural (SN) infection. At seven days of age, control and SA birds were inoculated with PBS or Typhimurium, respectively. Five SA birds were transferred to SN cages to facilitate natural infection. Cecal content and blood samples were collected at 0, 8, 14, and 21 days of age for microbiota and leukocyte analysis, respectively. A significant change in microbiota composition was observed in both groups as noted by a decrease in and and an increase in . Leukocyte analysis revealed a decrease in the percentage of circulating monocytes at 7 days post-infection while a decrease in thrombocyte and an increase in heterophil percentages were seen at 14 days post-infection. Taken together, these results demonstrate the ability of to modulate the intestinal microbiota to facilitate colonization. Additionally, results indicated an early role of monocytes and thrombocytes during colonization, followed by heterophils.
减少商业鸡群中的[病原体名称未明确]对降低因接触受污染的家禽及家禽产品而导致的人类沙门氏菌感染至关重要。由于肠道微生物群在预防病原体定植中发挥重要作用，我们试图了解[病原体名称未明确]感染与盲肠微生物群及宿主免疫系统之间的关系。孵化当天的肉鸡雏鸡被分为三种处理组：对照组、人工感染组（SA）和自然感染组（SN）。在7日龄时，对照组和SA组鸡分别接种磷酸盐缓冲液（PBS）或鼠伤寒沙门氏菌。将5只SA组鸡转移到SN组笼中以促进自然感染。分别在0、8、14和21日龄采集盲肠内容物和血液样本用于微生物群和白细胞分析。两组中微生物群组成均出现显著变化，表现为[某些细菌名称未明确]减少和[另一些细菌名称未明确]增加。白细胞分析显示，感染后7天循环单核细胞百分比降低，而感染后14天血小板减少且嗜异性粒细胞百分比增加。综上所述，这些结果证明了[病原体名称未明确]调节肠道微生物群以促进定植的能力。此外，结果表明单核细胞和血小板在定植过程中起早期作用，随后是嗜异性粒细胞。
