基于 HoO-TiO 纳米带电极的用于高选择性和高灵敏度检测癌症 miRNA 的方法。
HoO-TiO Nanobelts Electrode for Highly Selective and Sensitive Detection of Cancer miRNAs.
机构信息
School of Automation, Hangzhou Dianzi University, Hangzhou 310018, China.
Department of Chemistry and Biochemistry, University of California, 1156 High Street, Santa Cruz, CA 95064, USA.
出版信息
Biosensors (Basel). 2022 Sep 27;12(10):800. doi: 10.3390/bios12100800.
The design and engineering of effective electrode materials is critical in the development of electrochemical sensors. In the present study, HoO-TiO nanobelts were synthesized by an alkaline hydrothermal process. The structure and morphology were investigated by X-ray diffraction (XRD) and field emission scanning electron microscope (FESEM) measurements. The HoO-TiO nanobelts showed a distinctly enhanced (004) reflection peak and rough surfaces and were used for the electrochemical selective sensing of various cancer miRNAs, such as prostate cancer miR-141, osteosarcoma miR-21, and pancreatic cancer miR-1290. Voltammetric measurements showed an oxidation peak at +0.4, +0.2, and +1.53 V for the three different cancer biomarkers, respectively, with the detection limit as low as 4.26 aM. The results suggest that the HoO-TiO nanobelts can be used as active materials to detect early cancers, for in vitro screening of anticancer drugs, and molecular biology research.
在电化学传感器的发展中，有效电极材料的设计和工程至关重要。本研究通过碱性水热法合成了 HoO-TiO 纳米带。通过 X 射线衍射（XRD）和场发射扫描电子显微镜（FESEM）测量对其结构和形态进行了研究。HoO-TiO 纳米带表现出明显增强的（004）反射峰和粗糙表面，可用于电化学选择性感测各种癌症 miRNA，如前列腺癌 miR-141、骨肉瘤 miR-21 和胰腺癌 miR-1290。伏安测量显示三种不同癌症生物标志物的氧化峰分别在+0.4、+0.2 和+1.53 V，检测限低至 4.26 aM。结果表明，HoO-TiO 纳米带可用作活性材料来检测早期癌症、体外筛选抗癌药物和分子生物学研究。
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