Di Minno Alessandro, Aveta Achille, Gelzo Monica, Tripodi Lorella, Pandolfo Savio Domenico, Crocetto Felice, Imbimbo Ciro, Castaldo Giuseppe
Dipartimento di Farmacia, Università degli Studi di Napoli Federico II, 80131 Naples, Italy.
CEINGE-Biotecnologie Avanzate, 80131 Naples, Italy.
J Clin Med. 2022 Oct 17;11(20):6102. doi: 10.3390/jcm11206102.
Objective: Prostate cancer (PCa) is the most common type of cancer. Biomarkers help researchers to understand the mechanisms of disease and refine diagnostic panels. We measured urinary 8-hydroxy-2-deoxyguanosine (8-OHdG) and 8-iso-prostaglandin F2α (8-IsoF2α) to assess oxidative stress damage in PCa patients undergoing robot-assisted radical prostatectomy (RARP). Methods: Forty PCa patients were enrolled in the study. Urine was collected before (T0) and 3 months after the RARP procedure (T1). 8-OHdG and 8-IsoF2α were measured through liquid chromatography-tandem mass spectrometry. Sex- and age-matched healthy subjects served as controls (CTRL). Results: At T0, patients exhibited significantly higher levels of 8-OHdG than CTRL (p = 0.026). At T1, 23/40 patients who completed the 3-month follow-up showed levels of 8-OHdG that were significantly lower than at T0 (p = 0.042), and comparable to those of the CTRL subjects (p = 0.683). At T0, 8-Iso-PGF2α levels were significantly higher in PCa patients than in CTRL subjects (p = 0.0002). At T1, 8-Iso-PGF2α levels were significantly lower than at T0 (p < 0.001) and were comparable to those of CTRL patients (p = 0.087). Conclusions: A liquid chromatography-tandem mass spectrometry method reveals enhanced OHdG and 8-Iso-PGF2α in the urine of PCa patients. RARP normalizes such indices of oxidative stress. Large-sized sample studies and long-term follow-ups are now needed to validate these urinary biomarkers for use in the early prevention and successful treatment of PCa.
前列腺癌（PCa）是最常见的癌症类型。生物标志物有助于研究人员了解疾病机制并完善诊断指标。我们测量了接受机器人辅助根治性前列腺切除术（RARP）的PCa患者尿液中的8-羟基-2-脱氧鸟苷（8-OHdG）和8-异前列腺素F2α（8-IsoF2α），以评估氧化应激损伤。方法：40例PCa患者纳入本研究。在RARP手术前（T0）和术后3个月（T1）收集尿液。通过液相色谱-串联质谱法测量8-OHdG和8-IsoF2α。性别和年龄匹配的健康受试者作为对照（CTRL）。结果：在T0时，患者的8-OHdG水平显著高于CTRL（p = 0.026）。在T1时，完成3个月随访的40例患者中有23例的8-OHdG水平显著低于T0时（p = 0.042），且与CTRL受试者相当（p = 0.683）。在T0时，PCa患者的8-异前列腺素F2α水平显著高于CTRL受试者（p = 0.0002）。在T1时，8-异前列腺素F2α水平显著低于T0时（p < 0.001），且与CTRL患者相当（p = 0.087）。结论：液相色谱-串联质谱法显示PCa患者尿液中的OHdG和8-异前列腺素F2α升高。RARP使这些氧化应激指标恢复正常。现在需要进行大样本研究和长期随访，以验证这些尿液生物标志物在PCa早期预防和成功治疗中的应用。
