Satou Ryouichi, Iwasaki Miyu, Kamijo Hideyuki, Sugihara Naoki
Department of Epidemiology and Public Health, Tokyo Dental College, Tokyo 101-0061, Japan.
Department of Social Security for Dentistry, Tokyo Dental College, Tokyo 101-0061, Japan.
Materials (Basel). 2022 Oct 14;15(20):7171. doi: 10.3390/ma15207171.
In this study, we attempted to develop a dental caries prevention method using a bioapatite (BioHap), an eggshell-derived apatite with nanoparticle size and biocompatibility, with a high-concentration fluoride tooth surface application method. The enamel acid resistance after the application of the proposed method was compared with that of a conventional topical application of fluoride using bovine tooth enamel as an example. The tooth samples were divided into three groups based on the preventive treatment applied, and an acid challenge was performed. The samples were evaluated for acid resistance using qualitative and quantitative analytical methods. The BioHap group demonstrated reduced enamel loss and improved micro-Vickers hardness, along with a thick coating layer, decreased reaction area depth, and decreased mineral loss value and lesion depth. The combination of BioHap with high-concentration fluoride led to the formation of a thick coating layer on the enamel surface and better suppression of demineralization than the conventional method, both qualitatively and quantitatively. The proposed biocompatible nano-hydroxyapatite coating method is expected to become a new standard for providing professional care to prevent dental caries.
在本研究中，我们尝试开发一种龋齿预防方法，该方法使用生物磷灰石（BioHap），一种具有纳米颗粒尺寸和生物相容性的蛋壳衍生磷灰石，并采用高浓度氟化物牙齿表面涂抹法。以牛牙釉质为例，将所提出方法应用后的釉质耐酸性与传统局部应用氟化物的耐酸性进行比较。根据所应用的预防处理将牙齿样本分为三组，并进行酸蚀试验。使用定性和定量分析方法评估样本的耐酸性。BioHap组表现出釉质损失减少、显微维氏硬度提高，同时涂层增厚、反应区深度减小、矿物质损失值和病变深度降低。BioHap与高浓度氟化物的组合导致在釉质表面形成厚涂层，并且在定性和定量方面都比传统方法更好地抑制脱矿质作用。所提出的生物相容性纳米羟基磷灰石涂层方法有望成为预防龋齿专业护理的新标准。
