Alzoughool Foad, Al-Zghoul Mohammad Borhan, Ghanim Bayan Y, Atoum Manar, Aljawarneh Yousef, Idkaidek Nasir, Qinna Nidal A
Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, The Hashemite University, Zarqa 13133, Jordan.
Division of Health Sciences, Fujairah Women's College, Higher Colleges of Technology, Fujairah 1626, United Arab Emirates.
Metabolites. 2022 Oct 3;12(10):939. doi: 10.3390/metabo12100939.
Irisin is an exercise-induced myokine implicated as a fundamental mediator of physical activity benefits. The aim of the present study was to investigate the role of the chronic administration model of irisin on the physiological and molecular status of skeletal muscle. A total of 20 female Sprague Dawley rats (250 ± 40 g) were implanted with an irisin-loaded osmotic pump (5 µg/kg/day) for 42 days; in addition, 3 females received a single subcutaneous injection of irisin (5 µg/kg). On a weekly basis for six weeks, animals were weighed and blood samples were collected. After 42 days, hind muscle biopsies were collected for histology and gene analysis. Serum irisin, clinical biochemistry, and histopathology were quantified and evaluated. Genes encoding for different physiological muscle activities, such as oxidative stress, fatty acid metabolism, muscle hypertrophy, mitochondrial fusion, and aging were assayed. The results showed a significant reduction in body weight percentage and creatine kinase level without affecting the morphological characteristics of skeletal muscle. Significant changes were noted in genes involved in muscle physiological activity, growth, and aging, as well as genes encoding for the antioxidant system, fatty acid oxidation processes, and mitochondrial fusion. In conclusion, exogenous irisin can induce the same physiological and molecular mechanisms that might be induced by exercise.
鸢尾素是一种运动诱导产生的肌动蛋白，被认为是体育活动益处的基本介质。本研究的目的是探讨鸢尾素长期给药模型对骨骼肌生理和分子状态的作用。总共20只雌性斯普拉格-道利大鼠（250±40克）植入了负载鸢尾素的渗透泵（5微克/千克/天），持续42天；此外，3只雌性大鼠接受了一次鸢尾素皮下注射（5微克/千克）。在六周的时间里，每周对动物进行称重并采集血样。42天后，采集后肢肌肉活检样本进行组织学和基因分析。对血清鸢尾素、临床生化指标和组织病理学进行了量化和评估。检测了编码不同生理肌肉活动的基因，如氧化应激、脂肪酸代谢、肌肉肥大、线粒体融合和衰老相关基因。结果显示体重百分比和肌酸激酶水平显著降低，而不影响骨骼肌的形态特征。在参与肌肉生理活动、生长和衰老的基因，以及编码抗氧化系统、脂肪酸氧化过程和线粒体融合的基因中发现了显著变化。总之，外源性鸢尾素可以诱导与运动可能诱导的相同的生理和分子机制。
