Jiang Shiman, Chen Yanfei, Han Shengyi, Lv Longxian, Li Lanjuan
State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Centre for Infectious Diseases, Collaborative Innovation Centre for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Rd., Hangzhou 310003, China.
Jinan Microecological Biomedicine Shandong Laboratory, Jinan 250021, China.
Microorganisms. 2022 Sep 21;10(10):1882. doi: 10.3390/microorganisms10101882.
Next-generation sequencing (NGS) has become a widely used technology in biological research. NGS applications for clinical pathogen detection have become vital technologies. It is increasingly common to perform fast, accurate, and specific detection of clinical specimens using NGS. Pathogenic fungi with high virulence and drug resistance cause life-threatening clinical infections. NGS has had a significant biotechnological impact on detecting bacteria and viruses but is not equally applicable to fungi. There is a particularly urgent clinical need to use NGS to help identify fungi causing infections and prevent negative impacts. This review summarizes current research on NGS applications for fungi and offers a visual method of fungal detection. With the development of NGS and solutions for overcoming sequencing limitations, we suggest clinicians test specimens as soon as possible when encountering infections of unknown cause, suspected infections in vital organs, or rapidly progressive disease.
下一代测序（NGS）已成为生物学研究中广泛使用的技术。NGS在临床病原体检测中的应用已成为关键技术。使用NGS对临床标本进行快速、准确和特异性检测越来越普遍。具有高毒力和耐药性的致病真菌会导致危及生命的临床感染。NGS在检测细菌和病毒方面产生了重大的生物技术影响，但对真菌的适用性并不相同。临床上迫切需要利用NGS来帮助识别引起感染的真菌并防止负面影响。本综述总结了目前关于NGS在真菌检测中的应用研究，并提供了一种真菌检测的可视化方法。随着NGS的发展以及克服测序局限性的解决方案的出现，我们建议临床医生在遇到病因不明的感染、重要器官的疑似感染或快速进展性疾病时尽快对标本进行检测。
