Romano Ida, Vitiello Giuseppe, Gallucci Noemi, Di Girolamo Rocco, Cattaneo Andrea, Poli Annarita, Di Donato Paola
Institute of Biomolecular Chemistry, National Research Council of Italy, Via Campi Flegrei, 34, 80078 Pozzuoli, Italy.
Department of Chemical, Materials and Production Engineering, University of Naples Federico II, Piazzale V. Tecchio 80, 80125 Naples, Italy.
Microorganisms. 2022 Sep 21;10(10):1885. doi: 10.3390/microorganisms10101885.
The biogenic synthesis of nanomaterials, i.e., synthesis carried out by means of living organisms, is an emerging technique in nanotechnology since it represents a greener and more eco-friendly method for the production of nanomaterials. In this line, in order to find new biological entities capable of biogenic synthesis, we tested the ability of some extremophilic microorganisms to carry out the biogenic production of AgNPs and SeNPs. Silver NPs were produced extracellularly by means of the thermophilic strain SAMU; the haloalkaliphilic strain 5AG was instead found to be useful for the synthesis of SeNPs. The structural characterization of the biogenic nanoparticles showed that both the Ag and Se NPs possessed a protein coating on their surface and that they were organized in aggregates. Moreover, both types of NPs were found be able to exert an interesting antibacterial effect against either Gram-positive or Gram-negative species. This study confirmed that extremophilic microorganisms can be considered valuable producers of biologically active nanoparticles; nevertheless, further experiments must be performed to improve the synthesis protocols in addition to the downstream processes.
纳米材料的生物合成，即通过生物体进行的合成，是纳米技术中一种新兴的技术，因为它代表了一种更绿色、更环保的纳米材料生产方法。为此，为了找到能够进行生物合成的新生物实体，我们测试了一些极端微生物进行银纳米颗粒（AgNPs）和硒纳米颗粒（SeNPs）生物合成的能力。通过嗜热菌株SAMU在细胞外产生银纳米颗粒；相反，发现嗜盐碱菌株5AG可用于合成硒纳米颗粒。生物合成纳米颗粒的结构表征表明，银纳米颗粒和硒纳米颗粒表面均有一层蛋白质包膜，且它们聚集在一起。此外，发现这两种类型的纳米颗粒对革兰氏阳性或革兰氏阴性菌均具有有趣的抗菌作用。这项研究证实，极端微生物可被视为具有生物活性的纳米颗粒的有价值生产者；然而，除了下游工艺外，还必须进行进一步的实验以改进合成方案。
