Department of Food Engineering, Faculty of Chemical and Metallurgical Engineering, Istanbul Technical University, 34469 Istanbul, Turkey.
Institute of Nutritional Sciences, University of Hohenheim, Garbenstraße 28, 70599 Stuttgart, Germany.
Molecules. 2022 Oct 11;27(20):6807. doi: 10.3390/molecules27206807.
In this work, pressurized hot water extraction (PHWE) of hydrophilic polyphenols from black rosehip fruit was maximized using response surface methodology for simultaneous optimization in terms of extraction yield, total antioxidant capacity, total (poly)phenols, catechin, total monomeric anthocyanins, and cyanidin-3--glucoside. Extraction parameters, including temperature (X1: 40-80 °C) and the solvent-to-solid ratio (X2: 10-40 mL/g), were investigated as independent variables. Experimentally obtained values were fitted to a second-order polynomial model, and optimal conditions were determined using multiple regression analysis and analysis of variance. The black rosehip extract (BRE) obtained at optimized PHWE conditions was further encapsulated in biopolymer-coated liposomes and spray dried to enhance its processing and digestive stability. After reconstitution, the fabricated particles had an average size of 247-380 nm and a zeta-potential of 15-45 mV. Moreover, encapsulation provided remarkable protection of the phenolics under in vitro gastrointestinal digestion conditions, resulting in up to a 5.6-fold more phenolics in the bioaccessible fraction, which also had 2.9-8.6-fold higher antioxidant activity compared to the nonencapsulated BRE. In conclusion, PHWE in combination with a biopolymer coating is a potent method for the production of stable and safe edible natural extracts for the delivery of (poly)phenolic compounds in food and dietary supplements.
在这项工作中，采用响应面法对黑玫瑰果中亲水性多酚进行了加压热水提取（PHWE），以同时优化提取产率、总抗氧化能力、总（多）酚、儿茶素、总单体花青素和矢车菊素-3-O-葡萄糖苷。提取参数，包括温度（X1：40-80°C）和溶剂与固体的比例（X2：10-40 mL/g），作为自变量进行了研究。通过多元回归分析和方差分析对实验获得的值进行了拟合，确定了最佳条件。在优化的 PHWE 条件下获得的黑玫瑰果提取物（BRE）进一步包封在生物聚合物涂层脂质体中并喷雾干燥，以提高其加工和消化稳定性。再重构后，制得的颗粒平均粒径为 247-380nm，zeta 电位为 15-45mV。此外，包封在体外胃肠道消化条件下对酚类物质提供了显著的保护作用，使可生物利用部分的酚类物质增加了 5.6 倍，而生物可利用部分的抗氧化活性比未包封的 BRE 高 2.9-8.6 倍。总之，PHWE 与生物聚合物涂层相结合是一种生产稳定、安全的食用天然提取物的有效方法，可用于在食品和膳食补充剂中递送（多）酚类化合物。
