Molecular Physical-Chemistry R&D Unit, Department of Chemistry, University of Coimbra, Rua Larga, 3004-535 Coimbra, Portugal.
Centre for Functional Ecology, Department of Life Sciences, University of Coimbra, Calçada Martim de Freitas, 3000-456 Coimbra, Portugal.
Molecules. 2022 Oct 18;27(20):7006. doi: 10.3390/molecules27207006.
spp. are invasive in Southern Europe, and their high propagation rates produce excessive biomass, exacerbating wildfire risk. However, lignocellulosic biomass from spp. may be utilised for diverse biorefinery applications. In this study, attenuated total reflectance Fourier transform infrared spectroscopy (FTIR-ATR), high-performance anion-exchange chromatography pulsed amperometric detection (HPAEC-PAD) and lignin content determinations were used for a comparative compositional characterisation of . , . and . . Additionally, biomass was treated with three white-rot fungi species (, and ), which preferentially degrade lignin. Our results showed that the pre-treatments do not significantly alter neutral sugar composition while reducing lignin content. Sugar release from enzymatic saccharification was enhanced, in some cases possibly due to a synergy between white-rot fungi and mild alkali pretreatments. For example, in . stems treated with alkali and . , saccharification yield was 702.3 nmol mg, which is higher than the samples treated only with alkali (608.1 nmol mg), and 2.9-fold higher than the non-pretreated controls (243.9 nmol mg). By characterising biomass and pretreatments, generated data creates value for unused biomass resources, contributing to the implementation of sustainable biorefining systems. In due course, the generated value will lead to economic incentives for landowners to cut back invasive spp. more frequently, thus reducing excess biomass, which exacerbates wildfire risk.
spp. 在南欧具有入侵性，其高繁殖率产生了过多的生物量，加剧了野火风险。然而， spp. 的木质纤维素生物质可用于各种生物炼制应用。在这项研究中，使用衰减全反射傅里叶变换红外光谱（FTIR-ATR）、高效阴离子交换色谱脉冲安培检测（HPAEC-PAD）和木质素含量测定法对.,. 和.. 进行了比较组成特征化。此外，还对三种白腐真菌（, 和 ）进行了生物质处理，这些真菌优先降解木质素。我们的结果表明，预处理不会显著改变中性糖的组成，同时降低木质素的含量。酶解糖化过程中糖的释放得到了增强，在某些情况下，可能是由于白腐真菌和温和碱预处理之间的协同作用。例如，在经碱和. 预处理的. 茎中，糖化产率为 702.3 nmol mg，高于仅用碱处理的样品（608.1 nmol mg），比未经预处理的对照样品（243.9 nmol mg）高 2.9 倍。通过对生物质和预处理进行表征，生成的数据为未充分利用的生物质资源创造了价值，为可持续生物炼制系统的实施做出了贡献。随着时间的推移，所产生的价值将为土地所有者带来经济激励，使其更频繁地砍伐入侵性 spp.，从而减少加剧野火风险的过量生物量。
