Department of Pathology, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA.
Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA.
Nutrients. 2022 Oct 19;14(20):4384. doi: 10.3390/nu14204384.
Background Emerging research suggests hyperglycemia can increase intestinal permeability. Ginger and its bioactive compounds have been reported to benefit diabetic animals due to their anti-inflammatory and antioxidant properties. In this study, we revealed the beneficial effect of gingerol-enriched ginger (GEG) on intestinal health (i.e., barrier function, mitochondrial function, and anti-inflammation) in diabetic rats. Methods Thirty-three male Sprague Dawley rats were assigned to three groups: low-fat diet (control group), high-fat-diet (HFD) + streptozotocin (single low dose 35 mg/kg body weight (BW) after 2 weeks of HFD feeding) (DM group), and HFD + streptozotocin + 0.75% GEG in diet (GEG group) for 42 days. Glucose tolerance tests (GTT) and insulin tolerance tests (ITT) were conducted at baseline and prior to sample collection. Total pancreatic insulin content was determined by ELISA. Total RNA of intestinal tissues was extracted for mRNA expression using qRT-PCR. Results Compared to the DM group, the GEG group had improved glucose tolerance and increased pancreatic insulin content. Compared to those without GEG (DM group), GEG supplementation (GEG group) increased the gene expression of tight junction (Claudin-3) and antioxidant capacity (SOD1), while it decreased the gene expression for mitochondrial fusion (MFN1), fission (FIS1), biogenesis (PGC-1α, TFAM), mitophagy (LC3B, P62, PINK1), and inflammation (NF-κB). Conclusions Ginger root extract improved glucose homeostasis in diabetic rats, in part, via improving intestinal integrity and mitochondrial dysfunction of GI health.
新的研究表明高血糖会增加肠道通透性。姜及其生物活性化合物具有抗炎和抗氧化特性，已被报道可有益于糖尿病动物。在这项研究中，我们揭示了姜辣素富集姜（GEG）对糖尿病大鼠肠道健康（即屏障功能、线粒体功能和抗炎）的有益作用。
33 只雄性 Sprague Dawley 大鼠被分为三组：低脂饮食（对照组）、高脂肪饮食（HFD）+链脲佐菌素（HFD 喂养 2 周后单次低剂量 35mg/kg 体重（BW））（DM 组）和 HFD+链脲佐菌素+饮食中 0.75% GEG（GEG 组），共 42 天。在基线和取样前进行葡萄糖耐量试验（GTT）和胰岛素耐量试验（ITT）。通过 ELISA 测定胰岛总胰岛素含量。提取肠道组织总 RNA，用 qRT-PCR 检测 mRNA 表达。
与 DM 组相比，GEG 组的葡萄糖耐量得到改善，胰岛胰岛素含量增加。与未添加 GEG 的 DM 组（DM 组）相比，添加 GEG（GEG 组）增加了紧密连接（Claudin-3）和抗氧化能力（SOD1）的基因表达，同时降低了线粒体融合（MFN1）、分裂（FIS1）、生物发生（PGC-1α、TFAM）、自噬（LC3B、P62、PINK1）和炎症（NF-κB）的基因表达。
姜根提取物通过改善肠道完整性和胃肠道线粒体功能障碍，改善了糖尿病大鼠的葡萄糖稳态。
