Vuković Jovana S, Filipović Vuk V, Babić Radić Marija M, Vukomanović Marija, Milivojevic Dusan, Ilic-Tomic Tatjana, Nikodinovic-Runic Jasmina, Tomić Simonida Lj
University of Belgrade, Faculty of Technology and Metallurgy, Karnegijeva 4, 11000 Belgrade, Serbia.
University of Belgrade, Institute of Molecular Genetics and Genetic Engineering, Vojvode Stepe 444a, 11000 Belgrade, Serbia.
Polymers (Basel). 2022 Oct 21;14(20):4459. doi: 10.3390/polym14204459.
Scaffold hydrogel biomaterials designed to have advantageous biofunctional properties, which can be applied for controlled bioactive agent release, represent an important concept in biomedical tissue engineering. Our goal was to create scaffolding materials that mimic living tissue for biomedical utilization. In this study, two novel series of interpenetrating hydrogel networks (IPNs) based on 2-hydroxyethyl methacrylate/gelatin and 2-hydroxyethyl methacrylate/alginate were crosslinked using N-ethyl-N'-(3-dimethyl aminopropyl)carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS). Characterization included examining the effects of crosslinker type and concentration on structure, morphological and mechanical properties, in vitro swelling, hydrophilicity as well as on the in vitro cell viability (fibroblast cells) and in vivo () interactions of novel biomaterials. The engineered IPN hydrogel scaffolds show an interconnected pore morphology and porosity range of 62.36 to 85.20%, favorable in vitro swelling capacity, full hydrophilicity, and Young's modulus values in the range of 1.40 to 7.50 MPa. In vitro assay on healthy human fibroblast (MRC5 cells) by MTT test and in vivo () survival assays show the advantageous biocompatible properties of novel IPN hydrogel scaffolds. Furthermore, in vitro controlled release study of the therapeutic agent resveratrol showed that these novel scaffolding systems are suitable controlled release platforms. The results revealed that the use of EDC and the combination of EDC/NHS crosslinkers can be applied to prepare and tune the properties of the IPN 2-hydroxyethyl methacrylate/alginate and 2-hydroxyethyl methacrylate/gelatin hydrogel scaffolds series, which have shown great potential for biomedical engineering applications.
设计具有有利生物功能特性的支架水凝胶生物材料，可用于控制生物活性剂的释放，这是生物医学组织工程中的一个重要概念。我们的目标是创建模仿生物组织以供生物医学利用的支架材料。在本研究中，基于甲基丙烯酸2-羟乙酯/明胶和甲基丙烯酸2-羟乙酯/海藻酸盐的两个新型互穿水凝胶网络（IPN）系列，使用N-乙基-N'-(3-二甲基氨基丙基)碳二亚胺盐酸盐（EDC）和N-羟基琥珀酰亚胺（NHS）进行交联。表征包括研究交联剂类型和浓度对结构、形态和机械性能、体外溶胀、亲水性以及新型生物材料的体外细胞活力（成纤维细胞）和体内（）相互作用的影响。工程化的IPN水凝胶支架呈现出相互连通的孔形态，孔隙率范围为62.36%至85.20%，具有良好的体外溶胀能力、完全亲水性，杨氏模量值在1.40至7.50 MPa范围内。通过MTT试验对健康人成纤维细胞（MRC5细胞）进行的体外测定和体内（）存活试验表明，新型IPN水凝胶支架具有有利的生物相容性特性。此外，治疗剂白藜芦醇的体外控释研究表明，这些新型支架系统是合适的控释平台。结果表明，使用EDC以及EDC/NHS交联剂组合可用于制备和调节IPN甲基丙烯酸2-羟乙酯/海藻酸盐和甲基丙烯酸2-羟乙酯/明胶水凝胶支架系列的性能，这些支架系列在生物医学工程应用中显示出巨大潜力。
