Tuancharoensri Nantaprapa, Sonjan Sukhonthamat, Promkrainit Sudarat, Daengmankhong Jinjutha, Phimnuan Preeyawass, Mahasaranon Sararat, Jongjitwimol Jirapas, Charoensit Pensri, Ross Gareth M, Viennet Céline, Viyoch Jarupa, Ross Sukunya
Biopolymer Group, Department of Chemistry, Faculty of Science, Naresuan University, Phitsanulok 65000, Thailand.
Department of Pharmaceutical Technology, Center of Excellence for Innovation in Chemistry, Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok 65000, Thailand.
Polymers (Basel). 2023 Oct 11;15(20):4052. doi: 10.3390/polym15204052.
Tailored porous structures of poly(2-hydroxyethyl methacrylate) (PHEMA) and silk sericin (SS) were used to create porous hydrogel scaffolds using two distinct crosslinking systems. These structures were designed to closely mimic the porous nature of the native extracellular matrix. Conventional free radical polymerization of 2-hydroxyethyl methacrylate (HEMA) was performed in the presence of different concentrations of SS (1.25, 2.50, 5.00% /) with two crosslinking systems. A chemical crosslinking system with '-methylene bisacrylamide (MBAAm) and a physical crosslinking system with dimethylurea (DMU) were used: C-PHEMA/SS (crosslinked using MBAAm) and C-PHEMA/pC-SS (crosslinked using MBAAm and DMU). The focus of this study was on investigating the impact of these crosslinking methods on various properties of the scaffolds, including pore size, pore characteristics, polymerization time, morphology, molecular interaction, in vitro degradation, thermal properties, and in vitro cytotoxicity. The various crosslinked networks were found to appreciably influence the properties of the scaffolds, especially the pore sizes, in which smaller sizes and higher numbers of pores with high regularity were seen in C-PHEMA/1.25 pC-SS (17 ± 2 μm) than in C-PHEMA/1.25 SS (34 ± 3 μm). Semi-interpenetrating networks were created by crosslinking PHEMA-MBAAm-PHEMA while incorporating free protein molecules of SS within the networks. The additional crosslinking step involving DMU occurred through hydrogen bonding of the -C=O and -N-H groups with the SS, resulting in the simultaneous incorporation of DMU and SS within the PHEMA networks. As a consequence of this process, the scaffold C-PHEMA/pC-SS exhibited smaller pore sizes compared to scaffolds without DMU crosslinking. Moreover, the incorporation of higher loadings of SS led to even smaller pore sizes. Additionally, the gelation time of C-PHEMA/pC-SS was delayed due to the presence of DMU in the crosslinking system. Both porous hydrogel scaffolds, C-PHEMA/pC-SS and PHEMA, were found to be non-cytotoxic to the normal human skin dermal fibroblast cell line (NHDF cells). This promising result indicates that these hydrogel scaffolds have potential for use in tissue engineering applications.
聚甲基丙烯酸2-羟乙酯（PHEMA）和丝胶蛋白（SS）的定制多孔结构被用于通过两种不同的交联系统创建多孔水凝胶支架。这些结构旨在紧密模拟天然细胞外基质的多孔性质。在两种交联系统存在的情况下，以不同浓度的SS（1.25%、2.50%、5.00%）进行甲基丙烯酸2-羟乙酯（HEMA）的常规自由基聚合。使用了含N,N'-亚甲基双丙烯酰胺（MBAAm）的化学交联系统和含二甲基脲（DMU）的物理交联系统：C-PHEMA/SS（使用MBAAm交联）和C-PHEMA/pC-SS（使用MBAAm和DMU交联）。本研究的重点是研究这些交联方法对支架各种性能的影响，包括孔径、孔隙特征、聚合时间、形态、分子相互作用、体外降解、热性能和体外细胞毒性。发现各种交联网络对支架性能有显著影响，尤其是孔径，其中C-PHEMA/1.25 pC-SS（17±2μm）中可见的孔径比C-PHEMA/1.25 SS（34±3μm）更小且孔隙数量更多、规律性更高。通过交联PHEMA-MBAAm-PHEMA并在网络中掺入SS的游离蛋白质分子形成了半互穿网络。涉及DMU的额外交联步骤通过-C=O和-N-H基团与SS的氢键作用发生，导致DMU和SS同时掺入PHEMA网络中。由于这一过程，与未进行DMU交联的支架相比，支架C-PHEMA/pC-SS的孔径更小。此外，更高负载量SS的掺入导致孔径更小。此外，由于交联系统中存在DMU，C-PHEMA/pC-SS的凝胶化时间延迟。发现两种多孔水凝胶支架C-PHEMA/pC-SS和PHEMA对正常人皮肤成纤维细胞系（NHDF细胞）均无细胞毒性。这一有前景的结果表明这些水凝胶支架在组织工程应用中具有应用潜力。
Zotero 插件
