Babić Radić Marija M, Filipović Vuk V, Vuković Jovana S, Vukomanović Marija, Ilic-Tomic Tatjana, Nikodinovic-Runic Jasmina, Tomić Simonida Lj
University of Belgrade, Faculty of Technology and Metallurgy, Karnegijeva 4, 11000 Belgrade, Serbia.
University of Belgrade, Institute of Molecular Genetics and Genetic Engineering, Vojvode Stepe 444a, 11000 Belgrade, Serbia.
Polymers (Basel). 2023 Mar 25;15(7):1643. doi: 10.3390/polym15071643.
The idea of this study was to create a new scaffolding system based on 2-hydroxyethyl methacrylate, gelatin, and alginate that contains titanium(IV) oxide nanoparticles as a platform for the controlled release of the bioactive agent curcumin. The innovative strategy to develop hybrid scaffolds was the modified porogenation method. The effect of the scaffold composition on the chemical, morphology, porosity, mechanical, hydrophilicity, swelling, degradation, biocompatibility, loading, and release features of hybrid scaffolds was evaluated. A porous structure with interconnected pores in the range of 52.33-65.76%, favorable swelling capacity, fully hydrophilic surfaces, degradability to 45% for 6 months, curcumin loading efficiency above 96%, and favorable controlled release profiles were obtained. By applying four kinetic models of release, valuable parameters were obtained for the curcumin/PHEMA/gelatin/alginate/TiO release platform. Cytotoxicity test results depend on the composition of the scaffolds and showed satisfactory cell growth with visible cell accumulation on the hybrid surfaces. The constructed hybrid scaffolds have suitable high-performance properties, suggesting potential for further in vivo and clinical studies.
本研究的目的是创建一种基于甲基丙烯酸2-羟乙酯、明胶和藻酸盐的新型支架系统，该系统含有二氧化钛纳米颗粒，作为生物活性剂姜黄素的控释平台。开发混合支架的创新策略是改良的致孔方法。评估了支架组成对混合支架的化学、形态、孔隙率、机械性能、亲水性、溶胀性、降解性、生物相容性、负载和释放特性的影响。获得了具有52.33-65.76%相互连通孔隙的多孔结构、良好的溶胀能力、完全亲水的表面、6个月内降解至45%、姜黄素负载效率高于96%以及良好的控释曲线。通过应用四种释放动力学模型，获得了姜黄素/聚甲基丙烯酸羟乙酯/明胶/藻酸盐/二氧化钛释放平台的有价值参数。细胞毒性测试结果取决于支架的组成，显示出令人满意的细胞生长，在混合表面有可见的细胞积累。构建的混合支架具有合适的高性能特性，表明有进一步进行体内和临床研究的潜力。
