Meng Di, Wang Yuling, Liu Tongjun
Department of Stomatology, The Central Hospital Affilliated to Shandong First Medical University, Jinan, China.
Department of Stomatology, Shandong Qianfoshan Hospital, Jinan, China.
Front Chem. 2022 Oct 10;10:1019663. doi: 10.3389/fchem.2022.1019663. eCollection 2022.
Clinically, periodontitis is a chronic nonspecific inflammation that leads to damaged teeth and their supporting gum tissues. Although many studies on periodontitis have been conducted, therapy with natural products is still rare. Silibinin has been proven to have anti-inflammatory and antioxidant activities. However, the effects of silibinin on lipopolyssacharide (LPS)-induced inflammation in periodontal ligaments (PDLs) have not yet been investigated. In this study, the PDLs were treated with silibinin (10, 20, and 40 μM) in the presence of LPS. The results showed that silibinin treatment reduced the levels of NO, PGE, IL-6, TNF-α, MMP-1, and MMP-3 and enhanced the activities of superoxide dismutase (SOD) and glutathione (GSH). Moreover, silibinin treatment downregulated RANKL levels and upregulated OPG and ALP levels. In summary, silibinin protected PDLs against LPS-induced inflammation, oxidative stress, and osteogenic differentiation.
临床上，牙周炎是一种慢性非特异性炎症，会导致牙齿及其支持性牙龈组织受损。尽管已经对牙周炎进行了许多研究，但使用天然产物进行治疗仍然很少见。水飞蓟宾已被证明具有抗炎和抗氧化活性。然而，水飞蓟宾对脂多糖（LPS）诱导的牙周膜（PDL）炎症的影响尚未得到研究。在本研究中，在存在LPS的情况下，用不同浓度（10、20和40μM）的水飞蓟宾处理牙周膜。结果表明，水飞蓟宾处理降低了NO、PGE、IL-6、TNF-α、MMP-1和MMP-3的水平，并增强了超氧化物歧化酶（SOD）和谷胱甘肽（GSH）的活性。此外，水飞蓟宾处理下调了RANKL水平，上调了OPG和ALP水平。总之，水飞蓟宾可保护牙周膜免受LPS诱导的炎症、氧化应激和成骨分化的影响。
